Shrinking Fabrication of a Glucose-Responsive Glucagon Microneedle Patch.
Shrinking Fabrication of a Glucose-Responsive Glucagon Microneedle Patch.
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DOI：
10.1002/advs.202203274
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发表时间：
2022-10
期刊：
影响因子：
15.1
通讯作者：
Gu, Zhen
中科院分区：
文献类型：
--
作者：
Wang, Zejun;Fu, Ruxing;Han, Xiao;Wen, Di;Wu, Yifan;Li, Song;Gu, Zhen
一种以安全、非侵入性和葡萄糖响应性方式提供胰升糖素补充剂的微型设备是避免日常糖尿病治疗过程中胰岛素过量导致致命低血糖后果的理想选择。然而，模具辅助的生物医学材料或设备的微制造通常需要高分辨率的激光消融来缩小结构设计的规模。此外，大多数用于制造装置的聚合物药物输送材料在水环境中是可溶解或可变形的，这限制了形状固定后基于洗涤的清洁和纯化过程。这项研究利用了3D打印辅助模铸的设计灵活性，并提出了一种收缩微制造方法，允许随后的洗涤程序在聚合过程中去除有毒单体残留物。通过基质体积变化介导的释放动力学控制，开发了葡萄糖响应型经皮高血糖素微针贴剂，证明了该方法的可行性。在1型糖尿病小鼠模型中显示，这种经皮贴片可以逆转低血糖的发生，同时降低治疗期间单体残留物引起刺激的风险。从微观结构设计的成型分辨率限制中解脱出来，这种收缩方法进一步提供了对生物医学材料制造后净化的洞察。收缩微加工策略保证了聚合后单体残留物的充分净化，并利用脱水收缩来达到指定的设备尺寸。这种方法扩大了生物相容性较差的聚合物候选物库，用于生物医学目的。用这种方法产生了一种葡萄糖响应型胰升糖素微针贴片，用于处理低血糖紧急情况。
A microdevice that offers glucagon supplements in a safe, non‐invasive, and glucose‐responsive manner is ideal for avoiding fatal hypoglycemia consequences from insulin overdosage during daily diabetes treatment. However, mold‐assisted microfabrication of biomedical materials or devices typically needs high‐resolution laser ablation to scale down structural design. In addition, the majority of the polymeric drug delivery materials being used to fabricate devices are dissolvable or deformable in aqueous environments, which restricts washing‐based cleaning and purification procedures post shape fixation. This study leverages the design flexibility of 3D printing‐assisted mold casting and presents a shrinking microfabrication approach that allows subsequent washing procedures to remove toxic monomer residues during polymerization. The feasibility of this approach is demonstrated by developing a glucose‐responsive transdermal glucagon microneedle patch through matrix volume change‐mediated release kinetic control. Shown in the type 1 diabetic mouse model, this transdermal patch can reverse the occurrence of hypoglycemia while lowering the risk of monomer residue‐induced irritation during treatment. Freeing from the restrain of molding resolution for microstructure design, this shrinking methodology further provides an insight into post‐fabrication purifications of biomedical materials. The shrinking microfabrication strategy grants sufficient purification of monomer residues after polymerization and leverages dehydration shrinkage to achieve the designated device size. This approach expands the library of polymeric candidates that have less superior biocompatibility to be used for biomedical purposes. A glucose‐responsive glucagon microneedle patch is generated with this method for dealing with hypoglycemia emergencies.
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DOI：
10.1073/pnas.2011099117
发表时间：
2020-11-24
影响因子：
11.1
作者：
Wang, Zejun;Wang, Jinqiang;Gu, Zhen
通讯作者：
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影响因子：
20.6
作者：
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影响因子：
28.1
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DOI：
10.1002/smll.201603028
发表时间：
2017-05
期刊：
Small (Weinheim an der Bergstrasse, Germany)
影响因子：
--
作者：
Yu J;Zhang Y;Sun W;Kahkoska AR;Wang J;Buse JB;Gu Z
通讯作者：
Gu Z