Whole plant chamber to examine sensitivity of cereal gas exchange to changes in evaporative demand.
Whole plant chamber to examine sensitivity of cereal gas exchange to changes in evaporative demand.
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DOI：
10.1186/s13007-018-0357-9
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发表时间：
2018
期刊：
影响因子：
5.1
通讯作者：
Dodd IC
中科院分区：
文献类型：
--
作者：
Jauregui I;Rothwell SA;Taylor SH;Parry MAJ;Carmo-Silva E;Dodd IC
提高植物水分利用效率是提高作物产量对不利气候变化适应能力的主要目标。识别WUE的遗传变异通常依赖于光合作用（An）和蒸腾作用（Tr）的瞬时测量，或在叶片水平上的碳同位素鉴别的综合测量。然而，叶气体交换测量本身并不足以代表整个植物的反应，特别是如果周围的植物蒸发需求的变化。在这里，我们描述了一个整个工厂的气体交换系统，可以迅速改变蒸发的需求时，测量一个，Tr和内在的WUE（iWUE），并确定在此响应的遗传变异。在稳态条件下，N不受VPD的限制，但在高蒸发需求下，一些小麦品种限制Tr，从而提高iWUE。这些变化可能是ABA依赖的，因为大麦ABA缺陷突变体（Az 34）未能限制Tr高蒸发需求。尽管较高的Tr，Az 34表现出较低的An比野生型（WT）大麦，因为在Rubisco羧化活性的限制。外源ABA对Az 34的Tr和An的影响比WT大麦更敏感，ABA通过底物限制和Rubisco活性降低而抑制了Az 34的光合作用。 检查全植物对改变的VPD的气体交换响应可以鉴定全植物iWUE中的遗传变异，并促进对潜在机制的理解。本文的在线版本（10.1186/s13007-018-0357-9）包含补充材料，可供授权用户使用。
Improving plant water use efficiency (WUE) is a major target for improving crop yield resilience to adverse climate change. Identifying genetic variation in WUE usually relies on instantaneous measurements of photosynthesis (An) and transpiration (Tr), or integrative measurements of carbon isotope discrimination, at the leaf level. However, leaf gas exchange measurements alone do not adequately represent whole plant responses, especially if evaporative demand around the plant changes. Here we describe a whole plant gas exchange system that can rapidly alter evaporative demand when measuring An, Tr and intrinsic WUE (iWUE) and identify genetic variation in this response. An was not limited by VPD under steady-state conditions but some wheat cultivars restricted Tr under high evaporative demand, thereby improving iWUE. These changes may be ABA-dependent, since the barley ABA-deficient mutant (Az34) failed to restrict Tr under high evaporative demand. Despite higher Tr, Az34 showed lower An than wild-type (WT) barley because of limitations in Rubisco carboxylation activity. Tr and An of Az34 were more sensitive than WT barley to exogenous spraying with ABA, which restricted photosynthesis via substrate limitation and decreasing Rubisco activation. Examining whole plant gas exchange responses to altered VPD can identify genetic variation in whole plant iWUE, and facilitate an understanding of the underlying mechanism(s). The online version of this article (10.1186/s13007-018-0357-9) contains supplementary material, which is available to authorized users.
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影响因子：
6.2
作者：
Duursma, Remko A.;Barton, Craig V. M.;McMurtrie, Ross E.
通讯作者：
McMurtrie, Ross E.
影响因子：
7.3
作者：
Assmann, SM;Snyder, JA;Lee, YRJ
通讯作者：
Lee, YRJ
影响因子：
7.4
作者：
Cochard, H;Coll, L;Améglio, T
通讯作者：
Améglio, T
影响因子：
2.3
作者：
ALKHATIB, K;PAULSEN, GM
通讯作者：
PAULSEN, GM
影响因子：
7.4
作者：
Franks, Peter J.;Farquhar, Graham D.
通讯作者：
Farquhar, Graham D.