Comparative SILAC proteomic analysis of Trypanosoma brucei bloodstream and procyclic lifecycle stages.
Comparative SILAC proteomic analysis of Trypanosoma brucei bloodstream and procyclic lifecycle stages.
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DOI：
10.1371/journal.pone.0036619
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ferguson MA
中科院分区：
文献类型：
--
作者：
Urbaniak MD;Guther ML;Ferguson MA
原生动物寄生虫布氏锥虫在哺乳动物宿主和昆虫载体之间具有复杂的二代生命周期，并且其蛋白质组在生命周期阶段之间的适应对其生存和毒力至关重要。我们已经优化了一个程序，培养布氏锥虫原环型细胞的条件下，适合稳定同位素标记的氨基酸在文化（SILAC）和报告的比较蛋白质组学分析培养的原环型和血流型T。布氏细胞我们总共能够鉴定3959种蛋白质，并定量3553种蛋白质的SILAC比率，错误发现率为0.01。大量的蛋白质（10.6%）的差异调节更多的5倍之间的生命周期阶段，包括那些参与寄生虫的表面涂层，并在线粒体和糖体能量代谢。我们的蛋白质组学数据与转录组学研究基本一致，但在蛋白质水平上观察到的倍数变化明显大于mRNA水平。
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host and an insect vector, and adaption of its proteome between lifecycle stages is essential to its survival and virulence. We have optimized a procedure for growing Trypanosoma brucei procyclic form cells in conditions suitable for stable isotope labeling by amino acids in culture (SILAC) and report a comparative proteomic analysis of cultured procyclic form and bloodstream form T. brucei cells. In total we were able to identify 3959 proteins and quantify SILAC ratios for 3553 proteins with a false discovery rate of 0.01. A large number of proteins (10.6%) are differentially regulated by more the 5-fold between lifecycle stages, including those involved in the parasite surface coat, and in mitochondrial and glycosomal energy metabolism. Our proteomic data is broadly in agreement with transcriptomic studies, but with significantly larger fold changes observed at the protein level than at the mRNA level.
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