Human papillomavirus type 16 transformation of primary human embryonic fibroblasts requires expression of open reading frames E6 and E7
Human papillomavirus type 16 transformation of primary human embryonic fibroblasts requires expression of open reading frames E6 and E7
复制标题
人乳头瘤病毒 16 型转化原代人胚胎成纤维细胞需要表达开放阅读框 E6 和 E7
作者：
Sumie Watanabe;T. Kanda;K. Yoshiike
检测了人类乳头瘤病毒16型(HPV-16)早期基因转化原代人胚胎成纤维细胞(WI38)或延长成纤维细胞寿命的能力。通过新霉素筛选，将含有猴病毒40启动子控制的HPV-16亚基因组DNA片段转录单位的表达载体导入WI38细胞，并对长期培养的耐药细胞进行鉴定。含有完整E6E7开放阅读框(ORF)的片段能够延长WI38细胞的寿命。根据ORF移码突变的结果以及与含有E6和E7 ORF的单独质粒共转染获得的结果，得出结论：HPV-16转化(延长寿命)人成纤维细胞需要表达E6和E7基因。
Human papillomavirus 16 (HPV-16) early genes were tested for their ability to transform primary human embryonic fibroblasts (WI38) or to extend the life span of the fibroblasts. The expression plasmids containing the transcriptional unit for the HPV-16 subgenomic DNA fragments controlled by the simian virus 40 promoter were introduced into WI38 cells with the aid of neomycin selection, and the drug-resistant cells that survived long-term culture were characterized. The fragments containing E6E7 open reading frames (ORFs) intact were found to be capable of extending the life span of WI38 cells. From the results obtained with frameshift mutations in either ORF and from those obtained by cotransfection with the separate plasmids containing E6 and E7 ORFs, it was concluded that HPV-16 transformation (extension of the life span) of human fibroblasts requires expression of genes E6 and E7.
DOI：
10.1073/pnas.83.13.4680
发表时间：
1986-07-01
影响因子：
11.1
作者：
SMOTKIN, D;WETTSTEIN, FO
通讯作者：
WETTSTEIN, FO