A thermostable 5-enolpyruvylshikimate-3-phosphate synthase from Thermotoga maritima enhances glyphosate tolerance in Escherichia coli and transgenic Arabidopsis
A thermostable 5-enolpyruvylshikimate-3-phosphate synthase from Thermotoga maritima enhances glyphosate tolerance in Escherichia coli and transgenic Arabidopsis
复制标题
来自海栖热袍菌的热稳定性 5-烯醇丙酮莽草酸-3-磷酸合酶增强了大肠杆菌和转基因拟南芥中的草甘膦耐受性
DOI：
10.1007/s00792-019-01118-3
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发表时间：
2019-07
期刊：
影响因子：
2.9
通讯作者：
Yao Quanhong
中科院分区：
文献类型：
--
作者：
Wang Lijuan;Peng Rihe;Tian Yongsheng;Gao Jianjie;Wang Bo;Yao Quanhong
5-烯醇式莽草酸-3-磷酸合酶（EPSPS）的过表达，试图提供过量的EPSPS与草甘膦联合收割机结合，是提高植物草甘膦抗性的途径之一。极端微生物中的EPSPS是自然界为抵御进化压力而选择出来的，可能具有某些潜在的特异性生物学功能。在这项研究中，我们报告了一个新的II类EPSPS AroAT的克隆，表达和酶的特性。海栖热袍菌（Thermotoga maritimaMSB）8.该酶与其它EPSPS的序列同源性较低，是目前为止最耐热的EPSPS之一，其最适酶活为80 °C。该酶在50 °C以下和pH 4.0-10的范围内保持活性，这表明其在恶劣环境下，特别是在热带地区和碱性土壤中稳定。AroAT的Ki/Km值。maritimasugged表明，该酶表现出强大的竞争结合PEP对草甘膦的能力和高草甘膦耐受性。此外，aroAT。maritimagene转化拟南芥。转基因株系对15 mM草甘膦表现出抗性，证明了转基因株系在草甘膦抗性植物培育中的应用价值。
5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) overexpression, attempting to provide excess EPSPS to combine with glyphosate, is one way to improve glyphosate resistance of plants. The EPSPS in extremophiles which is selected by nature to withstand the evolutionary pressure may possess some potential-specific biological functions. In this study, we reported the cloning, expression and enzymatic characterization of a novel Class II EPSPS AroAT. maritimafromThermotoga maritimaMSB8. The enzyme showed low sequence identities with other EPSPSs, and was one of the most thermostable EPSPSs so far, which showed the optimum enzyme activity at 80 °C. The enzyme maintains the activity below 50 °C and in a wide range of pH 4.0–10, which indicated its stability under rough environment, especially in tropical regions and alkaline soil. ExcellentKi/Kmvalue of AroAT. maritimasuggested that the enzyme showed powerful competitive binding capacity of PEP over glyphosate and high glyphosate tolerance. Furthermore,aroAT. maritimagene was transformed intoArabidopsis thaliana. The transgenic lines were resistant to 15 mM glyphosate, which proved the application value in the cultivation of glyphosate-tolerant plants.
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影响因子：
56.9
作者：
COMAI, L;SEN, LC;STALKER, DM
通讯作者：
STALKER, DM
影响因子：
2.6
作者：
Hu Xu;A. Xiong;Wei Zhao;Yong-Sheng Tian;R. Peng;Jian-min Chen;Q. Yao
通讯作者：
Hu Xu;A. Xiong;Wei Zhao;Yong-Sheng Tian;R. Peng;Jian-min Chen;Q. Yao
DOI：
--
发表时间：
2006
期刊：
--
影响因子：
--
作者：
C. James
通讯作者：
C. James
影响因子：
7.4
作者：
Min Zhou;Honglin Xu;Xiaoli Wei;Zhiqiang Ye;Liping Wei;W. Gong;Yongqin Wang;Zhen Zhu
通讯作者：
Min Zhou;Honglin Xu;Xiaoli Wei;Zhiqiang Ye;Liping Wei;W. Gong;Yongqin Wang;Zhen Zhu
影响因子：
14.8
作者：
Zhang, Xiuren;Henriques, Rossana;Chua, Nam-Hai
通讯作者：
Chua, Nam-Hai