Induction of light emission by luminescent bacteria treated with UV light and chemical mutagens.
Induction of light emission by luminescent bacteria treated with UV light and chemical mutagens.
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经过紫外线和化学诱变剂处理的发光细菌诱导发光。
DOI：
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发表时间：
2002
影响因子：
2.4
通讯作者：
G. Węgrzyn
中科院分区：
文献类型：
--
作者：
A. Czyz;K. Plata;G. Węgrzyn
测试了发光细菌对紫外线和化学诱变剂的发光强度。我们证明，发光的六株海洋细菌（属于四个物种：发光杆菌leiognathi，P. phosphoreum，弧菌fischeri和V. harveyi）显着增加紫外线照射后不久稀释的文化。这种刺激的发光被取消在用氯霉素处理的细胞15分钟前UV照射，表明有效的基因表达是必要的UV介导的诱导发光。这些结果表明，在紫外线照射的细菌细胞的发光刺激可能独立的群体感应调节。在用化学诱变剂处理所有研究菌株的稀释培养物时也观察到显著的发光诱导：叠氮化钠（SA）、2-甲氧基-6-氯-9-（3-（2-氯乙基）氨基丙基氨基）吖啶x 2 HCl（ICR-191）、4-硝基-邻苯二胺（NPD）、4-硝基喹诺酮-N-氧化物（NQNO）、2-氨基芴（2-AF）和苯并[α]芘。这些结果支持了参与生物发光的基因属于SOS调节子的提议。根据这一建议，细菌发光系统在检测致突变化合物的试验中的使用进行了讨论。
Intensity of light emission by luminescent bacteria in response to UV irradiation and chemical mutagens was tested. We demonstrated that luminescence of six strains of marine bacteria (belonging to four species: Photobacterium leiognathi, P. phosphoreum, Vibrio fischeri and V. harveyi) is significantly increased by UV irradiation relatively shortly after dilution of cultures. Such a stimulation of luminescence was abolished in cells treated with chloramphenicol 15 min before UV irradiation, indicating that effective gene expression is necessary for UV-mediated induction of light emission. These results suggest that stimulation of luminescence in UV-irradiated bacterial cells may operate independently of the quorum sensing regulation. A significant induction of luminescence was also observed upon treatment of diluted cultures of all investigated strains with chemical mutagens: sodium azide (SA), 2-methoxy-6-chloro-9-(3-(2-chloroethyl)aminopropylamino)acridine x 2HCl (ICR-191), 4-nitro-o-phenylenediamine (NPD), 4-nitroquinolone-N-oxide (NQNO), 2-aminofluorene (2-AF), and benzo[alpha]pyrene. These results support the proposal that genes involved in bioluminescence belong to the SOS regulon. The use of bacterial luminescence systems in assays for detection of mutagenic compounds is discussed in the light of this proposal.
影响因子：
7.4
作者：
Zhang, XS;Rosenstein, BS;Wei, HC
通讯作者：
Wei, HC
DOI：
10.1002/bio.1170050205
发表时间：
1990-04
期刊：
Journal of bioluminescence and chemiluminescence
影响因子：
--
作者：
G. Shadel;J. Devine;T. Baldwin
通讯作者：
G. Shadel;J. Devine;T. Baldwin