Non-Psychoactive Cannabinoid Modulation of Nociception and Inflammation Associated with a Rat Model of Pulpitis.
Non-Psychoactive Cannabinoid Modulation of Nociception and Inflammation Associated with a Rat Model of Pulpitis.
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DOI：
10.3390/biom13050846
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发表时间：
2023-05-16
期刊：
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
尽管在牙科疼痛管理方面取得了进步，但紧急牙科护理最常见的原因之一是口腔面部疼痛。我们的研究旨在确定非精神活性大麻成分在治疗牙痛和相关炎症方面的效果。我们测试了两种非精神活性大麻成分大麻二酚(CbD)和β-石竹烯(β-CP)在与牙髓暴露相关的口面部疼痛的啮齿动物模型中的治疗潜力。用植物大麻素CBD(5 mg/kg ip)对SD大鼠进行假暴露或左侧下颌磨牙髓暴露。或倍半萜β-CP(30 mg/kg i.p.)在暴露前1h以及暴露后第1、3、7、10天给药。在牙髓暴露前和牙髓暴露后评估口腔面部机械性异位痛。第15天取三叉神经节进行组织学评价。牙髓暴露与同侧口面部区域和三叉神经节的显著口面部敏感和神经炎症相关。β-CP可显著降低口腔面部敏感度，而CBD不能。β-CP还显著降低炎症标志物AIF和CCL2的表达，而CBD仅降低AIF的表达。这些数据代表了第一个临床前证据，表明基于非精神活性大麻素的药物疗法可能为治疗与牙髓暴露相关的口面部疼痛提供治疗益处。
Despite advancements in dental pain management, one of the most common reasons for emergency dental care is orofacial pain. Our study aimed to determine the effects of non-psychoactive Cannabis constituents in the treatment of dental pain and related inflammation. We tested the therapeutic potential of two non-psychoactive Cannabis constituents, cannabidiol (CBD) and β-caryophyllene (β-CP), in a rodent model of orofacial pain associated with pulp exposure. Sham or left mandibular molar pulp exposures were performed on Sprague Dawley rats treated with either vehicle, the phytocannabinoid CBD (5 mg/kg i.p.) or the sesquiterpene β-CP (30 mg/kg i.p.) administered 1 h pre-exposure and on days 1, 3, 7, and 10 post-exposure. Orofacial mechanical allodynia was evaluated at baseline and post-pulp exposure. Trigeminal ganglia were harvested for histological evaluation at day 15. Pulp exposure was associated with significant orofacial sensitivity and neuroinflammation in the ipsilateral orofacial region and trigeminal ganglion. β-CP but not CBD produced a significant reduction in orofacial sensitivity. β-CP also significantly reduced the expression of the inflammatory markers AIF and CCL2, while CBD only decreased AIF expression. These data represent the first preclinical evidence that non-psychoactive cannabinoid-based pharmacotherapy may provide a therapeutic benefit for the treatment of orofacial pain associated with pulp exposure.
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影响因子：
4.3
作者：
de Melo Reis RA;Isaac AR;Freitas HR;de Almeida MM;Schuck PF;Ferreira GC;Andrade-da-Costa BLDS;Trevenzoli IH
通讯作者：
Trevenzoli IH
影响因子：
9.3
作者：
Dansereau MA;Midavaine É;Bégin-Lavallée V;Belkouch M;Beaudet N;Longpré JM;Mélik-Parsadaniantz S;Sarret P
通讯作者：
Sarret P
DOI：
10.1073/pnas.0803601105
发表时间：
2008-07-01
影响因子：
11.1
作者：
Gertsch, Juerg;Leonti, Marco;Zimmer, Andreas
通讯作者：
Zimmer, Andreas
DOI：
10.1016/s1079-2104(02)91732-4
发表时间：
2003-08-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Nusstein, JM;Beck, M
通讯作者：
Beck, M
影响因子：
1.6
作者：
Kalliomaki, Jarkko;Segerdahl, Marta;Quiding, Hans
通讯作者：
Quiding, Hans