The interferon-regulated gene signature is elevated in subacute cutaneous lupus erythematosus and discoid lupus erythematosus and correlates with the cutaneous lupus area and severity index score.
The interferon-regulated gene signature is elevated in subacute cutaneous lupus erythematosus and discoid lupus erythematosus and correlates with the cutaneous lupus area and severity index score.
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DOI：
10.1111/j.1365-2133.2012.10825.x
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发表时间：
2012-05
期刊：
影响因子：
--
通讯作者：
Werth VP
中科院分区：
文献类型：
--
作者：
Braunstein I;Klein R;Okawa J;Werth VP
在皮肤红斑狼疮（CLE）和系统性红斑狼疮（SLE）患者的血液和靶组织中，I型干扰素（IFN）调节蛋白的表达增加。SLE患者IFN调节的基因表达增加，这表明可能存在潜在的遗传缺陷。我们测量了CLE患者外周血中SLE中高表达的五种I型IFN调节基因的表达水平，并将其表达水平与皮肤疾病活动性相关。外周血来自10名健康对照和30名CLE患者，包括8名合并SLE患者。提取总RNA并逆转录成互补DNA。通过真实的时间PCR测量基因表达水平。将基因表达标准化为GAPDH，标准化为健康对照，然后求和以计算每个患者的IFN评分。用皮肤狼疮面积和严重程度指数（CLASI）评估疾病活动性。与健康对照组相比，亚急性皮肤型红斑狼疮（SCLE）和盘状红斑狼疮（DLE）患者的IFN评分升高，无论是否伴有SLE（SLE患者p< 0.01，无SLE患者p<0.05）。而肿胀型红斑狼疮（TLE）患者与健康对照组之间无差异。IFN评分与CLASI评分相关（斯皮尔曼Rho（r）= 0.55，p = 0.0017）。SCLE和DLE患者具有IFN特征，如在SLE中所见。基因表达水平与皮肤疾病活动相关。这些发现支持SLE和CLE的某些亚型之间的共同发病机制。
There is increased expression of type I interferon (IFN)-regulated proteins in the blood and target tissues of patients with cutaneous lupus erythematosus (CLE) and systemic lupus erythematosus (SLE). Patients with SLE have increased IFN-regulated gene expression pointing towards a possible underlying genetic defect. We measured expression levels of five type I IFN-regulated genes that are highly expressed in SLE in the peripheral blood of patients with CLE and correlated expression levels with cutaneous disease activity. Peripheral blood was obtained from 10 healthy controls and 30 patients with CLE, including 8 with concomitant SLE. Total RNA was extracted and reverse transcribed into complimentary DNA. Gene expression levels were measured by real time PCR. Gene expression was normalized to GAPDH, standardized to healthy controls and then summed to calculate an IFN score for each patient. Disease activity was assessed with the Cutaneous Lupus Area and Severity Index (CLASI). Patients with subacute cutaneous lupus erythematosus (SCLE) and discoid lupus erythematosus (DLE) had elevated IFN scores compared to healthy controls regardless of concomitant SLE (p< 0.01 with SLE and p<0.05 without SLE). There was no difference between patients with tumid lupus erythematosus (TLE) and healthy controls. The IFN score correlated with CLASI scores (Spearman’s Rho (r) = 0.55, p = 0.0017). Patients with SCLE and DLE have an IFN signature, as seen in SLE. The level of gene expression correlates with cutaneous disease activity. These findings support a shared pathogenesis between SLE and some subtypes of CLE.
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