Facilitation versus depression in cultured hippocampal neurons determined by targeting of Ca2+ channel Cavbeta4 versus Cavbeta2 subunits to synaptic terminals.
Facilitation versus depression in cultured hippocampal neurons determined by targeting of Ca2+ channel Cavbeta4 versus Cavbeta2 subunits to synaptic terminals.
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DOI：
10.1083/jcb.200702072
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发表时间：
2007-07-30
期刊：
影响因子：
--
通讯作者：
Herlitze S
中科院分区：
文献类型：
--
作者：
Xie M;Li X;Han J;Vogt DL;Wittemann S;Mark MD;Herlitze S
Ca2+通道β亚基决定了高压激活Ca2+通道复合物的运输和生理特性。我们对Cavβ亚基家族成员在海马神经元中的分布的分析表明，它们的突触分布与它们参与递质释放有关。我们发现，外源表达的Cavβ4b和Cavβ2a亚基呈簇状分布并定位于突触，而Cavβ1b和Cavβ3则均匀分布。根据其定位，Cavβ2a和Cavβ4b亚基调节自适应海马神经元的突触可塑性（即，在长时间刺激训练中，Cavβ2a诱导抑制，而Cavβ4b诱导配对脉冲促进[PPF]，随后诱导突触抑制）。Cavβ4b对PPF的诱导与释放概率和递质释放协同性的降低有关。这些结果表明，在突触前末端，Cavβ亚基以亚基特异性的方式决定了突触前Ca2+通道的门控特性，并可能与Ca2+通道的释放机制相关。
Ca2+ channel β subunits determine the transport and physiological properties of high voltage–activated Ca2+ channel complexes. Our analysis of the distribution of the Cavβ subunit family members in hippocampal neurons correlates their synaptic distribution with their involvement in transmitter release. We find that exogenously expressed Cavβ4b and Cavβ2a subunits distribute in clusters and localize to synapses, whereas Cavβ1b and Cavβ3 are homogenously distributed. According to their localization, Cavβ2a and Cavβ4b subunits modulate the synaptic plasticity of autaptic hippocampal neurons (i.e., Cavβ2a induces depression, whereas Cavβ4b induces paired-pulse facilitation [PPF] followed by synaptic depression during longer stimuli trains). The induction of PPF by Cavβ4b correlates with a reduction in the release probability and cooperativity of the transmitter release. These results suggest that Cavβ subunits determine the gating properties of the presynaptic Ca2+ channels within the presynaptic terminal in a subunit-specific manner and may be involved in organization of the Ca2+ channel relative to the release machinery.
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影响因子：
4.8
作者：
Chien, AJ;Gao, TY;Hosey, MM
通讯作者：
Hosey, MM
影响因子：
16.2
作者：
Han, Jing;Mark, Melanie D.;Herlitze, Stefan
通讯作者：
Herlitze, Stefan
影响因子：
64.5
作者：
Berggren, PO;Yang, SN;Flockerzi, V
通讯作者：
Flockerzi, V
DOI：
10.1073/pnas.051628998
发表时间：
2001-04-10
影响因子：
11.1
作者：
Herlitze, S;Zhong, HJ;Catterall, WA
通讯作者：
Catterall, WA
DOI：
10.1073/pnas.94.4.1512
发表时间：
1997-02-18
影响因子：
11.1
作者：
Herlitze, S;Hockerman, GH;Catterall, WA
通讯作者：
Catterall, WA