Molecular Cloning, Structure, and Expression of Mouse Estrogen-responsive Finger Protein Efp
Molecular Cloning, Structure, and Expression of Mouse Estrogen-responsive Finger Protein Efp
复制标题
小鼠雌激素响应指蛋白 Efp 的分子克隆、结构和表达
DOI：
--
复制
发表时间：
1995
影响因子：
4.8
通讯作者：
M. Muramatsu
中科院分区：
文献类型：
--
作者：
A. Orimo;S. Inoue;K. Ikeda;S. Noji;M. Muramatsu
我们以前已经发现了一个人类雌激素反应基因，EFP(雌激素反应手指蛋白)，它编码一个可能的转录调节因子(井上，S.，Orimo，A.，Hosoi，T.，Kondo，S.，Toyota oshima，H.，Kondo，T.，Ikegami，A.，Ouchi，Y.，Orimo，H.和Muramatsu，M.(1993)proc.娜塔莉。阿卡德。SCI。美国A·90,11117-11121)。在这里，我们报道了小鼠EFP基因的分离及其结构，它包含三个富含半胱氨酸的结构域(环指和B1和B2盒)、一个盘绕结构域和一个C-末端结构域。核糖核酸酶保护实验检测到子宫、卵巢和胎盘中EFP基因的高水平表达。通过原位杂交组织化学方法，在子宫、乳腺、卵巢和脑中也检测到了EFP的转录本，并且EFP和雌激素受体mRNA在这些雌性器官中的共同定位表现得尤为明显。此外，作为雌激素靶器官的子宫和脑中的EFP mRNA水平在体内被17β-雌二醇上调。此外，通过整体原位杂交，EFP和雌激素受体mRNA均在11.5天胚胎的脑泡中染色。这些发现提出了EFP是一种雌激素反应基因的可能性，它在不同的靶器官中介导雌激素的作用。
We have previously identified a human estrogen-responsive gene, efp (estrogen-responsive finger protein), which encodes a putative transcription regulator (Inoue, S., Orimo, A., Hosoi, T., Kondo, S., Toyoshima, H., Kondo, T., Ikegami, A., Ouchi, Y., Orimo, H., and Muramatsu, M.(1993) Proc. Natl. Acad. Sci. U. S. A. 90, 11117-11121). Here, we report isolation of mouse Efp cDNA and its structure containing three cysteine-rich domains (RING finger and B1 and B2 boxes), a coiled-coil domain, and a C-terminal domain. High levels of Efp mRNA were detected in uterus, ovary, and placenta by RNase protection assay. By in situ hybridization histochemistry the transcripts of efp were also detected in uterus, mammary gland, ovary, and brain, and the co-localization of Efp and estrogen receptor mRNA was particularly demonstrated in these female organs. Moreover, the level of Efp mRNA in uterus and brain, which are known as target organs for estrogen, was up-regulated in vivo by 17β-estradiol. Furthermore, both the Efp and estrogen receptor mRNA were stained in the brain vesicles of 11.5-day embryos by whole mount in situ hybridization. These findings raise the possibility that efp is an estrogen-responsive gene that mediates estrogen action in various target organs.
登录
查看更多内容
影响因子：
15.9
作者：
CHAN, EKL;HAMEL, JC;TAN, EM
通讯作者：
TAN, EM
影响因子：
158.5
作者：
STAMPFER, MJ;WILLETT, WC;HENNEKENS, CH
通讯作者：
HENNEKENS, CH
双免疫荧光法检测兔子宫雌、孕激素受体的差异分布。
影响因子：
4.8
作者：
Zaino,RJ;Clarke,CL;Feil,PD;Satyaswaroop,PG
通讯作者：
Satyaswaroop,PG
影响因子：
158.5
作者：
SMITH, EP;BOYD, J;KORACH, KS
通讯作者：
KORACH, KS
影响因子：
2.7
作者：
COX, KH;DELEON, DV;ANGERER, RC
通讯作者：
ANGERER, RC