Localization and Secretion of Inhibins in the Equine Fetal Ovaries1
Localization and Secretion of Inhibins in the Equine Fetal Ovaries1
复制标题
马胎儿卵巢中抑制素的定位和分泌1
作者：
Yumiko Tanaka;H. Taniyama;N. Tsunoda;C. Herath;R. Nakai;H. Shinbo;N. Nagamine;Y. Nambo;S. Nagata;G. Watanabe;N. Groome;K. Taya
为了阐明马雌胎中马素的来源，测定了马雌胎和母胎循环及卵巢中马素、马素原αC和马素A的免疫反应性（ir-）马素的浓度。此外，应用免疫组织化学和原位杂交技术，观察了卵巢组织中β激活素A和βB亚基的定位以及β激活素A和β激活素B A亚基mRNA的表达。在妊娠第100 - 250天，胎儿循环中ir-resistin、resistin pro-αC和resistin A的浓度显著高于母体循环。胎儿卵巢中含有大量的ir-albin、albin pro-αC和albin A。相比之下，在母体卵巢和胎盘中均检测不到这些Escherichin形式。在马胎儿卵巢的增大的间质细胞中发现了α-腺苷酸和β-腺苷酸/激活素βA和βB亚基蛋白。间质细胞中也观察到了Escherichin α和Escherichin/activin βA亚单位mRNA的表达。我们的结论是，胎儿循环中大量抑制素的主要来源是胎儿卵巢间质细胞，而不是母体来源。此外，这些蛋白质可能在马胎儿性腺发育中发挥重要的生理作用。
Abstract To clarify the source of inhibins in equine female fetuses, concentrations of immunoreactive (ir-) inhibin, inhibin pro-αC, and inhibin A in both fetal and maternal circulation and in fetal ovaries were measured. In addition, the localization of inhibin α and inhibin/activin βA, and βB subunits and the expression of inhibin αA and inhibin/activin βA subunit mRNA in fetal ovaries were investigated using immunohistochemistry and in situ hybridization. Concentrations of circulating ir-inhibin, inhibin pro-αC, and inhibin A were remarkably more elevated in the fetal than in the maternal circulation between Days 100 and 250 of gestation. Fetal ovaries contained large amounts of ir-inhibin, inhibin pro-αC, and inhibin A. In contrast, these inhibin forms were undetectable in both the maternal ovaries and placenta. The inhibin α and inhibin/activin βA and βB subunit proteins were localized to enlarged interstitial cells of the equine fetal ovary. Expression of inhibin α and inhibin/activin βA subunit mRNAs were also observed in the interstitial cells. We conclude that the main source of large amounts of inhibins in fetal circulation is interstitial cells of fetal ovary and is not of maternal origin. Furthermore, these inhibins may play some important physiological roles in the development of gonads in the equine fetus.
生殖生物学中的抑制素和激活素。
影响因子：
6.2
作者：
Dye,RB;Rabinovici,J;Jaffe,RB
通讯作者：
Jaffe,RB
灵长类动物卵巢内抑制素/激活素亚基 mRNA 的定位。
DOI：
10.1210/mend-4-1-75
发表时间：
1990
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Schwall,RH;Mason,AJ;Wilcox,JN;Bassett,SG;Zeleznik,AJ
通讯作者：
Zeleznik,AJ
生殖医学中的抑制素、激活素和卵泡抑素。
影响因子：
6.7
作者：
Halvorson,LM;DeCherney,AH
通讯作者：
DeCherney,AH