A parallel microfluidic flow cytometer for high-content screening.
A parallel microfluidic flow cytometer for high-content screening.
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DOI:
10.1038/nmeth.1595
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发表时间:
2011-05
期刊:
影响因子:
48
通讯作者:
Ehrlich, Daniel J.
中科院分区:
文献类型:
--
作者:
McKenna, Brian K.;Evans, James G.;Cheung, Man Ching;Ehrlich, Daniel J.
A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count 1-D imaging with flow cytometry. The 384 parallel flow channels of the PMC decouple count rate from signal-to-noise ratio. Using 6-pixel 1-D images, we investigated protein-localization in a yeast model for a human protein-misfolding disease and demonstrated the feasibility of a nuclear-translocation assay in Chinese-hamster-ovary (CHO) cells expressing a NFκB-GFP reporter.
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