Cationic silanes stabilize intermediates in DNA condensation
Cationic silanes stabilize intermediates in DNA condensation
复制标题
阳离子硅烷稳定 DNA 缩合中的中间体
DOI：
10.1016/s0014-5793(99)01237-5
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发表时间：
1999
期刊：
影响因子：
3.5
通讯作者：
J. Hoh
中科院分区：
文献类型：
--
作者：
Ye Fang;J. Hoh
DNA的体外浓缩已被广泛研究，以深入了解DNA在生物系统（如染色体和噬菌体头）中的压缩机制，并已被用于生产具有新型材料和功能特性的纳米结构颗粒。本文报道了阳离子硅烷在水溶液中对DNA的缩聚，它结合了多胺的缩聚特性和硅烷的交联化学性质。DNA可以用这些试剂可逆地凝聚成经典的环状和棒状结构。在低硅烷浓度下，DNA形成各种具有明确特征的环状结构，包括花形和香肠形结构。这些结构表明，在低硅烷浓度下，DNA-DNA之间的接触是稳定的，在这种接触中，dna链彼此之间的角度很大。观察到的这些结构的变化作为硅烷浓度的函数提示了环状体和棒状体形成的可能途径。
In vitro condensation of DNA has been widely studied to gain insight into the mechanisms of DNA compaction in biological systems such as chromosomes and phage heads and has been used to produce nanostructured particles with novel material and functional properties. Here we report on the condensation of DNA in aqueous solutions by cationic silanes, which combine the condensing properties of polyamines with the cross-linking chemistry of silanes. DNA can be reversibly condensed into classical toroidal and rod-shaped structures with these agents. At low silane concentrations DNA forms a variety of looped structures with well-defined characteristics, including flower- and sausage-shaped forms. These structures suggest that at low silane concentrations a DNA-DNA contact in which the strands are at very large angles to each other is stabilized. Changes in these structures observed as a function of silane concentration suggest possible pathways for the formation of toroids and rods.
影响因子：
5.6
作者：
WIDOM, J;BALDWIN, RL
通讯作者：
BALDWIN, RL
影响因子：
3.4
作者：
S. Leuba;Carlos Bustamante;Carlos Bustamante;K. Holde;Jordanka J. Zlatanova;Jordanka J. Zlatanova
通讯作者：
S. Leuba;Carlos Bustamante;Carlos Bustamante;K. Holde;Jordanka J. Zlatanova;Jordanka J. Zlatanova
DOI：
10.1073/pnas.84.21.7413
发表时间：
1987-11-01
影响因子：
11.1
作者：
FELGNER, PL;GADEK, TR;DANIELSEN, M
通讯作者：
DANIELSEN, M