Disentangling environmental drivers of circadian metabolism in desert-adapted mice.
Disentangling environmental drivers of circadian metabolism in desert-adapted mice.
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DOI：
10.1242/jeb.242529
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发表时间：
2021-09-15
期刊：
影响因子：
--
通讯作者：
MacManes MD
中科院分区：
文献类型：
--
作者：
Colella JP;Blumstein DM;MacManes MD
代谢是一种复杂的表型，由自然环境节律以及行为，形态和生理适应形成。代谢在历史上一直在恒定的环境条件下进行研究，但新的连续代谢表型分析方法现在提供了一个窗口，了解生物体对动态环境的反应，并能够识别非生物控制和相对于环境变化的生理反应的时间。我们使用间接量热法来表征适应沙漠的仙人掌小鼠（Peromyscus eremicus）的代谢表型，以响应模拟其原生环境的可变环境条件，而不是在恒定的温暖和恒定的凉爽条件下记录的那些，具有恒定的光周期和充分获得资源。我们发现了显著的两性异形，男性比女性更容易脱水。在昼夜环境变化下，大多数代谢变化发生在物理环境变化之前，并且在两种恒定处理下时间被打乱。产生的CO2消耗的O2（呼吸商）的比例达到大于1.0，只有在光照阶段在昼夜变化的条件下，一种模式，强烈表明，脂肪生成有助于能源和内源性水的生产。我们的研究结果是一致的，在这个物种的昼夜节律迟钝的历史描述（白天迟钝，晚上活跃），但拒绝迟钝是由食物限制或负水平衡的假设。总结：沙漠适应仙人掌小鼠（Peromyscus eremicus）的连续代谢表型确定性别和昼夜节律模式与脂肪生成和环境夹带一致的显着代谢差异。
Metabolism is a complex phenotype shaped by natural environmental rhythms, as well as behavioral, morphological and physiological adaptations. Metabolism has been historically studied under constant environmental conditions, but new methods of continuous metabolic phenotyping now offer a window into organismal responses to dynamic environments, and enable identification of abiotic controls and the timing of physiological responses relative to environmental change. We used indirect calorimetry to characterize metabolic phenotypes of the desert-adapted cactus mouse (Peromyscus eremicus) in response to variable environmental conditions that mimic their native environment versus those recorded under constant warm and constant cool conditions, with a constant photoperiod and full access to resources. We found significant sexual dimorphism, with males being more prone to dehydration than females. Under circadian environmental variation, most metabolic shifts occurred prior to physical environmental change and the timing was disrupted under both constant treatments. The ratio of CO2 produced to O2 consumed (the respiratory quotient) reached greater than 1.0 only during the light phase under diurnally variable conditions, a pattern that strongly suggests that lipogenesis contributes to the production of energy and endogenous water. Our results are consistent with historical descriptions of circadian torpor in this species (torpid by day, active by night), but reject the hypothesis that torpor is initiated by food restriction or negative water balance. Summary: Continuous metabolic phenotyping of desert-adapted cactus mice (Peromyscus eremicus) identifies significant metabolic differences between sexes and circadian patterning consistent with lipogenesis and environmental entrainment.
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DOI：
10.1016/j.bbalip.2014.11.008
发表时间：
2015-06
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Bridges D;Saltiel AR
通讯作者：
Saltiel AR
影响因子：
2.1
作者：
Breheny, Patrick;Burchett, Woodrow
通讯作者：
Burchett, Woodrow
影响因子：
2.8
作者：
Chappell, MA;Garland, T;Gomes, FR
通讯作者：
Gomes, FR
影响因子：
2.4
作者：
Alonso, Juan C.;Salgado, Ivan;Palacin, Carlos
通讯作者：
Palacin, Carlos
影响因子：
29
作者：
Adamovich Y;Rousso-Noori L;Zwighaft Z;Neufeld-Cohen A;Golik M;Kraut-Cohen J;Wang M;Han X;Asher G
通讯作者：
Asher G