Influence of flight on protein catabolism, especially myofilament breakdown, in homing pigeons
Influence of flight on protein catabolism, especially myofilament breakdown, in homing pigeons
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飞行对信鸽蛋白质分解代谢的影响，特别是肌丝分解
DOI：
10.1007/s003600050007
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
E. Haase
中科院分区：
文献类型：
--
作者：
R. Bordel;E. Haase
摘要为了研究归航过程中蛋白质的降解，建立了一种高效液相色谱法定量分析Nτ-甲基组氨酸的方法。其次，有必要证实Nτ-甲基组氨酸的排泄与家鸽肌丝断裂相关。在这些实验中，10只鸡皮下注射Nτ-[14 C]甲基组氨酸，并定量收集排泄物1周。在回收的94.5%放射性中，87.1%与Nτ-[14 C]甲基组氨酸相关，6.1%与N-乙酰基-Nτ-[14 C]甲基组氨酸相关。未代谢的Nτ-[14 C]甲基组氨酸的快速排泄验证了Nτ-甲基组氨酸排泄量是信鸽肌丝分解的一种量度的假设。在从368-646公里以外的释放地点飞行后，确定了耐力飞行对蛋白质分解的影响。返回后，血浆尿素和尿酸水平立即升高，而血浆Nτ-甲基组氨酸浓度与未飞行的对照鸡相比保持不变。与对照组相比，飞行鸽在飞行后24 h内排出的尿素和Nτ-甲基组氨酸显著增加，在飞行后96 h内排出的尿素和尿酸显著增加。我们的研究结果支持这一假设，即在信鸽蛋白质catalysts增加，在耐力飞行。Nτ-甲基组氨酸排泄增加可能是受损肌纤维修复过程的结果，包括肌丝的分解。
Abstract In order to study protein degradation during flight in homing, a high-performance liquid chromatography technique was developed for the quantitative analysis of Nτ-methylhistidine. Secondly, it was necessary to confirm that the excretion of Nτ-methylhistidine correlates with myofilament breakdown in homing pigeons. In these experiments, ten birds were subcutaneously injected with Nτ-[14C]methylhistidine and the excreta were quantitatively collected for 1 week. Of the 94.5% radioactivity recovered, 87.1% was associated with Nτ-[14C]methylhistidine and 6.1% with N-acetyl-Nτ-[14C]methylhistidine. This rapid excretion of unmetabolized Nτ-[14C]methylhistidine validates the assumption that the amount of Nτ-methylhistidine excreted is a measure of myofilament catabolism in homing pigeons. The influence of endurance flight on protein breakdown was determined after flights from release sites 368–646 km away. Immediately after return, plasma urea and uric acid levels were increased, whereas plasma concentration of Nτ-methylhistidine remained unchanged compared to unflown control birds. Flown pigeons excreted significantly more urea and Nτ-methylhistidine within 24 h and significantly more urea and uric acid within 96 h after flight than unflown controls. Our findings support the hypothesis that in homing pigeons protein catabolism is increased during endurance flight. Elevated Nτ-methylhistidine excretion probably results from repair processes in damaged muscle fibers, including breakdown of myofilaments.
人类抗阻运动后肌肉蛋白质周转率和氨基酸转运率增加。
DOI：
10.1152/ajpendo.1995.268.3.e514
发表时间：
1995
期刊：
The American journal of physiology.
影响因子：
--
作者：
Biolo,G;Maggi,SP;Williams,BD;Tipton,KD;Wolfe,RR
通讯作者：
Wolfe,RR