Serum Containing Tao-Hong-Si-Wu Decoction Induces Human Endothelial Cell VEGF Production via PI3K/Akt-eNOS Signaling.
Serum Containing Tao-Hong-Si-Wu Decoction Induces Human Endothelial Cell VEGF Production via PI3K/Akt-eNOS Signaling.
复制标题
桃红四物汤含血清通过 PI3K/Akt-eNOS 信号诱导人内皮细胞 VEGF 产生
DOI：
10.1155/2013/195158
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Han L
中科院分区：
文献类型：
--
作者：
Yin D;Liu Z;Peng D;Yang Y;Gao X;Xu F;Han L
桃红四物汤是我国著名的传统中药，在临床上被广泛应用于缺血性疾病的治疗。大鼠经口给予TSD（1.6 g/kg），每日2次，连续3天，制备TSD含药血清。TSD含药血清诱导人脐静脉内皮细胞增殖、VEGF分泌和一氧化氮（NO）生成。TSD的上述促凋亡作用可分别被PI 3 K抑制剂LY 294002或eNOS抑制剂L-NAME部分抑制，而LY 294002和L-NAME同时处理可完全抑制TSD的上述促凋亡作用。Western blot分析结果进一步表明，TSD含药血清上调HUVECs p-Akt和p-eNOS表达，LY 294002和L-NAME可显著抑制TSD含药血清上调p-Akt和p-eNOS表达，LY 294002和L-NAME可完全抑制TSD含药血清上调p-Akt和p-eNOS表达，提示TSD含药血清通过PI 3 K/Akt-eNOS信号通路诱导HUVECs VEGF表达。超高效液相色谱法检测TSD含药血清中含有羟丙基黄色素A、阿魏酸和川芎内酯，说明TSD含药血清的这些作用可能与这3种活性成分在血清中的吸收有关。
Tao-Hong-Si-Wu decoction (TSD) is a famous traditional Chinese medicine (TCM) and widely used for ischemic disease in China. TSD medicated serum was prepared after oral administration of TSD (1.6 g/kg) twice a day for 3 days in rats. TSD medicated serum induced human umbilical vein endothelial cells (HUVECs) proliferation, VEGF secretion, and nitric oxide (NO) production. These promoted effects of TSD were partly inhibited by treatment with PI3K inhibitor (LY294002) or eNOS inhibitor (L-NAME), respectively, and completely inhibited by treatment with LY294002 and L-NAME simultaneously. Western blot analysis findings further indicated that TSD medicated serum upregulated p-Akt and p-eNOS expressions, which were significantly inhibited by LY294002 or L-NAME and completely inhibited by both LY294002 and L-NAME; these results indicated that TSD medicated serum induced HUVECs VEGF expression via PI3K/Akt-eNOS signaling. TSD medicated serum contains hydroxysafflor yellow A, ferulic acid, and ligustilide detected by UPLC with standards, so these effect of TSD medicated serum may be associated with these three active compounds absorbed in serum.
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DOI：
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发表时间：
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影响因子：
2.9
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