Human pituitary tissue secretes a potent growth factor for chondrocyte proliferation.
Human pituitary tissue secretes a potent growth factor for chondrocyte proliferation.
复制标题
人类垂体组织分泌一种有效的软骨细胞增殖生长因子。
DOI：
10.1210/jcem-62-1-70
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
H. Friesen
中科院分区：
文献类型：
--
作者：
S. Kasper;H. Friesen
我们报告分泌从人垂体瘤碎片在器官培养的一个强大的有丝分裂原软骨细胞增殖。从肢端肥大症、泌乳素瘤和无功能腺瘤患者的垂体碎片中建立原代人垂体细胞和器官培养物。条件培养基含有刺激兔胎儿软骨细胞增殖的促有丝分裂因子，当在10%胎牛血清存在下加入Ham’s F-10培养基中时，导致细胞数量增加高达8倍。众所周知，腺瘤切除术后漏入手术区域的血液含有非常高浓度的垂体激素。从垂体手术部位收集的静脉“渗出物”中获得的血清样品也被发现含有软骨细胞生长促进活性。一些静脉血清样品以剂量依赖性方式刺激软骨细胞增殖，低至1微升血清的1：10稀释，表明分泌的物质确实非常有效。Sephadex G-100凝胶过滤和分析凝胶等电聚焦的培养基或血清样品从垂体窝表明，分泌的生长因子的垂体瘤片段，以及从静脉血清是相似的，如果不是相同的，软骨细胞生长因子（摩尔重量，43，000; pI 7.6-7.9）纯化从人垂体收集尸检。这些结果表明，软骨细胞生长促进因子（S）可能不仅分泌垂体瘤片段，但正常人垂体组织以及。
We report the secretion from human pituitary tumor fragments in organ culture of a potent mitogen for chondrocyte proliferation. Primary human pituitary cell and organ cultures were established from pituitary fragments obtained from patients with acromegaly, prolactinomas, and nonfunctional adenomas. The conditioned culture medium contained a mitogenic factor(s) that stimulated rabbit fetal chondrocyte proliferation, causing up to an 8-fold increase in cell number when added to Ham's F-10 medium in the presence of 10% fetal bovine serum. Blood leaking into the surgical field after the adenomectomy is known to contain very high concentrations of pituitary hormones. Serum samples, obtained from this venous "ooze" collected at the site of pituitary surgery, also were found to contain chondrocyte growth-promoting activity. Some venous serum samples stimulated chondrocyte proliferation in a dose-dependent manner down to a 1:10 dilution of 1 microliter serum, indicating that the material being secreted was very potent indeed. Gel filtration on Sephadex G-100 and analytical gel isoelectric focusing of culture media or serum samples from the pituitary fossa demonstrated that the growth factor secreted from the pituitary tumor fragments as well as from the venous serum is similar, if not identical, to chondrocyte growth factor (mol wt, 43,000; pI 7.6-7.9) purified from human pituitaries collected at autopsy. These results suggest that the chondrocyte growth-promoting factor(s) may not only be secreted by pituitary tumor fragments but by normal human pituitary tissue as well.
人类垂体血管床中的降钙素样免疫反应性。
DOI：
10.1210/jcem-53-5-985
发表时间：
1981
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Samaan,NA;Leavens,ME
通讯作者：
Leavens,ME
肢端肥大症患者独特的生长因子。
DOI：
10.1210/jcem-57-2-272
发表时间：
1983
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Ashcraft,MW;Hartzband,PI;VanHerle,AJ;Bersch,N;Golde,DW
通讯作者：
Golde,DW
DOI：
10.1073/pnas.81.3.935
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DERCOLE, AJ;STILES, AD;UNDERWOOD, LE
通讯作者：
UNDERWOOD, LE