The role ofSaccharomyces cerevisiae Cdc40p in DNA replication and mitotic spindle formation and/or maintenance
The role ofSaccharomyces cerevisiae Cdc40p in DNA replication and mitotic spindle formation and/or maintenance
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酿酒酵母 Cdc40p 在 DNA 复制和有丝分裂纺锤体形成和/或维持中的作用
DOI：
10.1007/bf00705642
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Y. Kassir
中科院分区：
文献类型：
--
作者：
N. Vaisman;A. Tsouladze;K. Robzyk;S. Ben;M. Kupiec;Y. Kassir
Successful progression through the cell cycle requires the coupling of mitotic spindle formation to DNA replication. In this report we present evidence suggesting that, inSaccharomyces cerevisiae, theCDC40 gene product is required to regulate both DNA replication and mitotic spindle formation. The deduced amino acid sequence ofCDC40 (455 amino acids) contains four copies of a β-transducin-like repeat. Cdc40p is essential only at elevated temperatures, as a complete deletion or a truncated protein (deletion of the C-terminal 217 amino acids in thecdc40-1 allele) results in normal vegetative growth at 23°C, and cell cycle arrest at 36°C. In the mitotic cell cycle Cdc40p is apparently required for at least two steps: (1) for entry into S phase (neither DNA synthesis, nor mitotic spindle formation occurs at 36°C and (2) for completion of S-phase (cdc40::LEU2 cells cannot complete the cell cycle when returned to the permissive temperature in the presence of hydroxyurea). The role of Cdc40p as a regulatory protein linking DNA synthesis, spindle assembly/maintenance, and maturation promoting factor (MPF) activity is discussed.
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DOI：
10.1073/pnas.88.21.9578
发表时间：
1991-11-01
影响因子：
11.1
作者：
CHIEN, CT;BARTEL, PL;FIELDS, S
通讯作者：
FIELDS, S
酿酒酵母 Cdc6 刺激 Abf1 DNA 结合活性。
DOI：
10.1074/jbc.273.3.1298
发表时间：
1998
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Feng,L;Wang,B;Jong,A
通讯作者：
Jong,A
影响因子：
3.3
作者：
T. Weinert;L. Hartwell
通讯作者：
T. Weinert;L. Hartwell
影响因子：
11.4
作者：
ROWLEY, R;SUBRAMANI, S;YOUNG, PG
通讯作者：
YOUNG, PG
影响因子：
10.5
作者：
WEINERT, TA;KISER, GL;HARTWELL, LH
通讯作者：
HARTWELL, LH