PCR SCREENING OF THE WOLBACHIA IN SOME ARTHROPODS AND NEMATODES IN KHUZESTAN PROVINCE
PCR SCREENING OF THE WOLBACHIA IN SOME ARTHROPODS AND NEMATODES IN KHUZESTAN PROVINCE
复制标题
胡齐斯坦省部分节肢动物和线虫中沃尔巴克氏体的 PCR 筛选
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
R. Hossein
中科院分区：
文献类型：
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作者：
P. Pourali;M. Ardakani;A. Jeloudar;R. Hossein
摘要 沃尔巴克氏体是一种必需的细胞内 α-变形菌，它感染一些节肢动物和线虫种群的生殖和体细胞组织。由于没有任何关于伊朗是否存在这种细菌的数据，本研究旨在确定 30 种节肢动物和线虫中是否存在这种细菌。从样品中提取 DNA 后，我们筛选了沃尔巴克氏菌属 (Wolbachia spp)。使用PCR方法用特异性引物。总共对 770 种节肢动物（22 属）和 41 种线虫（6 属）进行了沃尔巴克氏体筛查。总共有 7 个属的 167 个节肢动物样本（18 个菌落）和 1 个线虫样本呈阳性。 16S rDNA基因PCR阳性产物经RsaI酶切，确定沃尔巴克氏体类型为沃尔巴克氏体A超群。
Summary Wolbachia is an obligatory, intracellular α-proteobacterium which infect the reproductive and somatic tissues of some arthropod and nematode populations. Because there are not any available data on the presence of this bacterium in Iran, the present study was done to determine the presence of this bacterium among 30 species of arthropods and nematodes. After DNA extraction from samples, we screened Wolbachia spp. with specific primers using PCR method. A total of 770 arthropods (of 22 genera) and 41 nematodes (of 6 genera) were screened for Wolbachia. Overall 167 arthropod samples (18 colonies) from 7 genera and 1 nematode sample were found positive. Positive PCR products of 16S rDNA gene were digested with RsaI restriction enzyme and the types of Wolbachia were recognized as A supergroup of Wolbachia.
影响因子：
3.8
作者：
Dobson, SL;Bourtzis, K;O'Neill, SL
通讯作者：
O'Neill, SL