A competitive complex formation mechanism underlies trichome patterning on Arabidopsis leaves.
A competitive complex formation mechanism underlies trichome patterning on Arabidopsis leaves.
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DOI：
10.1038/msb.2008.54
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发表时间：
2008
影响因子：
9.9
通讯作者：
Hulskamp, Martin
中科院分区：
文献类型：
--
作者：
Digiuni, Simona;Schellmann, Swen;Geier, Florian;Greese, Bettina;Pesch, Martina;Wester, Katja;Dartan, Burcu;Mach, Valerie;Srinivas, Bhylahalli Purushottam;Timmer, Jens;Fleck, Christian;Hulskamp, Martin
拟南芥中的毛状体图案形成是植物中从头图案形成的模型系统。它被认为是典型的理论激活剂-抑制剂机制，尽管这一假设从未受到实验和理论相结合的方法的挑战。通过整合毛状体图案化系统的关键遗传和分子数据，我们开发了一种新的理论模型，该模型允许直接测试实验干预的效果和图案化表型的预测。我们的实验表明，毛状体抑制剂TRIPTYCHON转录激活已知的正调节GLABRA 1和GLABRA 3。此外，我们通过用GFP粒子轰击蛋白质融合物证明了TRIPTYCHON和CAPRICE而不是GLABRA 1和GLABRA 3可以在细胞之间移动。最后，理论上的考虑表明启动子交换和基础过表达实验，通过这些实验，我们能够区分毛状体图案模型的三种生物学上有意义的变体。我们的研究表明，理论和实验之间的相互作用可以揭示生物化学机制如何驱动生物模式化过程的新水平。
Trichome patterning in Arabidopsis serves as a model system for de novo pattern formation in plants. It is thought to typify the theoretical activator–inhibitor mechanism, although this hypothesis has never been challenged by a combined experimental and theoretical approach. By integrating the key genetic and molecular data of the trichome patterning system, we developed a new theoretical model that allows the direct testing of the effect of experimental interventions and in the prediction of patterning phenotypes. We show experimentally that the trichome inhibitor TRIPTYCHON is transcriptionally activated by the known positive regulators GLABRA1 and GLABRA3. Further, we demonstrate by particle bombardment of protein fusions with GFP that TRIPTYCHON and CAPRICE but not GLABRA1 and GLABRA3 can move between cells. Finally, theoretical considerations suggest promoter swapping and basal overexpression experiments by means of which we are able to discriminate three biologically meaningful variants of the trichome patterning model. Our study demonstrates that the mutual interplay between theory and experiment can reveal a new level of understanding of how biochemical mechanisms can drive biological patterning processes.
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影响因子：
56.9
作者：
MITCHISON, GJ
通讯作者：
MITCHISON, GJ
影响因子：
64.5
作者：
HULSKAMP, M;MISERA, S;JURGENS, G
通讯作者：
JURGENS, G
影响因子：
9.2
作者：
Berger, F;Haseloff, J;Dolan, L
通讯作者：
Dolan, L
影响因子：
4.6
作者：
Bernhardt, C;Zhao, MZ;Schiefelbein, J
通讯作者：
Schiefelbein, J
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF