Investigation of Optimized Treatment Conditions for Acoustic-Transfection Technique for Intracellular Delivery of Macromolecules.
Investigation of Optimized Treatment Conditions for Acoustic-Transfection Technique for Intracellular Delivery of Macromolecules.
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DOI：
10.1016/j.ultrasmedbio.2017.11.005
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发表时间：
2018-03
影响因子：
2.9
通讯作者：
Shung KK
中科院分区：
文献类型：
--
作者：
Kim MG;Yoon S;Chiu CT;Shung KK
通过在细胞内引入遗传物质来操纵细胞功能和结构一直是生物医学中最突出的研究领域之一。高频超声的声转染技术最近被发展起来，并通过在单个细胞水平上将小分子递送到HeLa细胞中以获得高细胞活力而得到证实。在我们证明了声转染技术的概念之后，对不同人类癌细胞系之间的处理条件进行了深入的研究，以进一步发展声转染作为一种通用的和适应性强的转染方法，通过满足高递送效率和细胞膜通透性的要求，以最小的膜破坏。为了确定不同细胞系的最佳处理条件，我们基于处理4小时和20小时后的递送效率、细胞膜渗透性和细胞活力开发了定量细胞内递送评分（IDS）。在优化的处理条件下，成功地实现了大分子的胞内递送和两种分子的同时胞内递送。我们证明DNA质粒通过声转染技术被递送到上胚层干细胞中，其表达瞬时mCherry荧光。
Manipulation of cellular functions and structures by introducing genetic materials inside cells has been one of the most prominent research areas in biomedicine. Acoustic-transfection with high frequency ultrasound has recently been developed and confirmed by intracellular delivery of small molecules into HeLa cell at single-cell level with high cell viability. After we proved the concept of acoustic-transfection technique, intensive investigation of treatment conditions between different human cancer cell lines has been performed to further develop acoustic-transfection as a versatile and adaptable transfection method by satisfying requirements of high delivery efficiency and cell membrane permeability with minimal membrane disruption. To determine optimal treatment conditions for different cell lines, we developed a quantitative intracellular delivery score (IDS) based on delivery efficiency, cell membrane permeability and cell viability after 4 and 20 hours of treatment. The intracellular delivery of macromolecule and simultaneous intracellular delivery of two molecules with optimal treatment conditions were successfully achieved. We demonstrated that DNA plasmid was delivered by acoustic-transfection technique into epiblast stem cells, which expressed transient mCherry fluorescence.
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影响因子：
4.2
作者：
Kim MG;Yoon S;Kim HH;Shung KK
通讯作者：
Shung KK
影响因子：
13.6
作者：
Chen ZG
通讯作者：
Chen ZG
影响因子：
4.3
作者：
Kim, Tae Kyung;Eberwine, James H.
通讯作者：
Eberwine, James H.
影响因子：
2.9
作者：
Karshafian, Raffi;Bevan, Peter D.;Burns, Peter N.
通讯作者：
Burns, Peter N.
影响因子：
23.9
作者：
Kim D;Kim CH;Moon JI;Chung YG;Chang MY;Han BS;Ko S;Yang E;Cha KY;Lanza R;Kim KS
通讯作者：
Kim KS