Comparing electroantennogram and behavioral responses of two Pseudacteon phorid fly species to body extracts of Black, Red and Hybrid imported fire ants, Solenopsis spp.
Comparing electroantennogram and behavioral responses of two Pseudacteon phorid fly species to body extracts of Black, Red and Hybrid imported fire ants, Solenopsis spp.
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比较两种 Pseudaacteon phorid 蝇种对黑色、红色和混合进口火蚁（Solenopsis spp）身体提取物的触角电图和行为反应。
DOI：
10.1016/j.jinsphys.2012.07.011
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发表时间：
2012-10
影响因子：
2.2
通讯作者：
Chen, Li
中科院分区：
文献类型：
--
作者：
Chen, Li
几种蚤蝇物种被引入美国南部，用于生物控制入侵的红火蚁，红火蚁（Solenopsis richteri）（黑色），红火蚁（红色），以及它们的杂交种。richteri×S. invicta（Hybrid）.根据先前的研究，亚瓜留纳型的三尖拟acteon tricuspis和台湾型的弯曲拟acteon curvatus可以区分三种火蚁，并对混合型和红火蚁有更大的偏好。我们推测，蚤蝇可能使用主机衍生的化学线索，以区分蚂蚁物种。为了确定蚤蝇对不同种类火蚁可能的嗅觉敏感性差异，我们比较了三尖棘蚁和弯曲棘蚁对黑、红和杂交火蚁身体提取物的触角电位和行为反应。作为工蚁，黑蚁和杂交火蚁的工蚁个体数远大于红火蚁在0.01、0.1、1只工蚁当量剂量下，黑、红、杂三种工蚁的平均体重分别为1.707、0.863、1.223mg/只，黑火蚁和杂交火蚁的身体提取物引起的EAG反应显著高于红火蚁。同样，雌性弯曲拟单胞菌对黑火蚁体提取物的EAG反应显著大于对红火蚁体提取物的EAG反应。为了消除工蚁大小对蚤蝇EAG反应的影响，我们进行了第二次EAG研究，使用1 mg蚂蚁当量（1 mg工蚁的身体提取物）。在EAG反应中没有记录到从相同数量的工人之间的三种火蚁（我们认为可行的杂交火蚁作为一个物种在本文中）的身体提取物的差异，这表明工人的大小差异在第一EAG研究中的EAG反应的差异。在两项EAG研究中，雄性三尖棘胸蚁对所有三种火蚁的EAG反应均显著高于雄性弯曲胸蚁。在四向嗅觉仪生物测定中，所有三种火蚁物种的工蚁体提取物对三尖蚁和弯曲蚁具有相同的吸引力（即两种蚤蝇物种在三种火蚁物种中没有显示出任何偏好）。总之，EAG和行为研究的结果表明，寄生蚤蝇利用宿主衍生的非极性化合物从工人蚂蚁提取出的己烷主机位置，但不是主机的偏好，因为这两个苍蝇物种是不能区分的三个火蚁物种的身体提取物。未来的研究将调查极性化合物和/或非化学线索可能参与介导蚤蝇的宿主偏好。
Several phorid fly species were introduced to the southern United States for biological control of the invasive imported fire ants, Solenopsis richteri (Black), Solenopsis invicta (Red), and their Hybrid S. richteri×S. invicta (Hybrid). It has been previously reported that the Jaguariuna biotype of Pseudacteon tricuspis and the Formosan biotype of Pseudacteon curvatus could distinguish among the three fire ant species with greater preference for Hybrid and Red fire ants. We hypothesized that phorid flies might use host derived chemical cues to differentiate ant species. To determine possible differential olfactory sensitivity of phorid fly species to different fire ant species, we compared electroantennogram (EAG) and behavioral responses of both sexes of P. tricuspis and P. curvatus to body extracts of Black, Red and Hybrid fire ants. As worker sizes of Black and Hybrid fire ants used in this study were much larger than that of Red fire ant (the average weight for Black, Red and Hybrid workers was 1.707, 0.863, 1.223mg per ants, respectively), at doses of 0.01, 0.1, 1 worker equivalent, body extracts of Black and Hybrid fire ant elicited significantly greater EAG response in both sexes of P. tricuspis than that of Red fire ant. Similarly, the EAG response in female P. curvatus to body extract of Black fire ant was significantly greater than to body extract of Red fire ant. To eliminate worker size influence on EAG response in phorid flies, we conducted a second EAG study using a dose of 1mg ant equivalent (body extract from 1mg of worker). No difference in EAG responses was recorded to body extract obtained from the same amount of workers among the three fire ant species (we consider viable Hybrid fire ant as a species in this paper), suggesting that worker size differences contributed to difference in EAG response in the first EAG study. In both EAG studies, male P. tricuspis showed significantly greater EAG responses than male P. curvatus to all three fire ant species. In four-way olfactometer bioassay, worker body extracts of all three fire ant species were equally attractive to P. tricuspis and P. curvatus (i.e. both phorid fly species did not show any preferences among the three fire ant species). Together, the results of the EAG and behavior studies suggest that parasitic phorid flies utilize host derived non-polar compounds from worker ants extracted out by hexane for host location but not for host preference, since both fly species are not able to distinguish among the body extracts of the three fire ant species. Future study will investigate possible involvement of polar compounds and/or non-chemical cues in mediating host preference by phorid flies.
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影响因子：
2.3
作者：
S. Porter;R. K. Meer;M. Pesquero;S. Campiolo;H. Fowler
通讯作者：
S. Porter;R. K. Meer;M. Pesquero;S. Campiolo;H. Fowler
影响因子：
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作者：
Chen, Li;Sharma, Kavita R.;Fadamiro, Henry Y.
通讯作者：
Fadamiro, Henry Y.
影响因子：
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作者：
R. J. Vázquez;S. Porter;J. Briano
通讯作者：
R. J. Vázquez;S. Porter;J. Briano
影响因子：
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作者：
N. J. Mehdiabadi;Elizabeth A. Kawazoe;L. Gilbert
通讯作者：
N. J. Mehdiabadi;Elizabeth A. Kawazoe;L. Gilbert
从火蚁 Solenopsis invicta 中分离出一种吡嗪报警信息素成分
影响因子：
2.3
作者：
Meer, Robert K. Vander;Preston, Catherine A.;Choi, Man-Yeon
通讯作者：
Choi, Man-Yeon