Genetically-engineered mesenchymal stem cells transfected with human HCN1 gene to create cardiac pacemaker cells
Genetically-engineered mesenchymal stem cells transfected with human HCN1 gene to create cardiac pacemaker cells
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基因工程间充质干细胞转染人HCN1基因以产生心脏起搏细胞
DOI：
10.1177/0300060513501123
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发表时间：
2013-10
影响因子：
1.6
通讯作者：
Jiang, Wen-Ping
中科院分区：
文献类型：
--
作者：
Yang, Xiang-Jun;Li, Hong-Xia;Han, Lian-Huan;Jiang, Wen-Ping
目的验证转染人超极化激活环核苷酸门控通道1（hHCN 1）基因的基因工程间充质干细胞（MSCs）能否成为心脏起搏细胞。方法采用慢病毒介导的方法将hHCN 1基因转染MSCs。采用全细胞膜片钳技术记录转染hHCN 1的MSCs表达的起搏电流（If）。通过将MSCs与新生兔心室肌细胞（NRVM）共培养来测定转染hHCN 1的MSCs对心肌细胞兴奋性的影响。采用全细胞电流钳技术记录NRVM的自发动作电位。结果转染hHCN 1的MSCs可检测到高水平的时间和电压依赖性内向超极化电流，该电流可被4 mM氯化铯抑制，提示HCN 1蛋白在MSCs中起If通道的作用。与转染pcDNA 3质粒对照的对照MSC共培养的NRVM的平均± SE搏动频率为82 ± 8次/min（n = 5），而与转染hHCN 1的MSC共培养的NRVM的平均± SE搏动频率为129 ± 11次/min（n = 5）。结论转染hHCN 1基因的基因工程MSCs可被修饰为心脏起搏细胞。
Objective To test the proof-of-principle that genetically-engineered mesenchymal stem cells (MSCs) transfected with the human hyperpolarization-activated cyclic nucleotide-gated channel 1 (hHCN1) gene can be modified to become cardiac pacemaker cells. Methods MSCs were transfected with the hHCN1 gene using lentiviral-based transfection. The expressed pacemaker current (If) in hHCN1-transfected MSCs was recorded using whole-cell patch-clamp analysis. The effect of the hHCN1-transfected MSCs on cardiomyocyte excitability was determined by coculturing the MSCs with neonatal rabbit ventricular myocytes (NRVM). The spontaneous action potentials of the NRVM were recorded by whole-cell current-clamp analysis. Results A high level time- and voltage-dependent inward hyperpolarization current that was inhibited by 4 mM caesium chloride was detected in hHCN1-transfected MSCs, suggesting that the HCN1 proteins acted as If channels in MSCs. The mean ± SE beating frequency in NRVMs cocultured with control MSCs transfected with the pcDNA3 plasmid control was 82 ± 8 beats/min (n = 5) compared with 129 ± 11 beats/min (n = 5) in NRVMs cocultured with hHCN1-transfected MSCs. Conclusions Genetically-engineered MSCs transfected with the hHCN1 gene can be modified to become cardiac pacemaker cells.
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影响因子：
4.7
作者：
Yafeng Zhou;Xiang-jun Yang;Hong-Xia Li;Lian-hua Han;Wen-ping Jiang
通讯作者：
Yafeng Zhou;Xiang-jun Yang;Hong-Xia Li;Lian-hua Han;Wen-ping Jiang
DOI：
10.1111/j.1540-8167.1995.tb00394.x
发表时间：
1995-12
影响因子：
2.7
作者：
D. DiFrancesco
通讯作者：
D. DiFrancesco
影响因子：
6.1
作者：
Min Tong;Xiang-jun Yang;Bao-yu Geng;Lian-hua Han;Yafeng Zhou;Xin Zhao;Hong-Xia Li
通讯作者：
Min Tong;Xiang-jun Yang;Bao-yu Geng;Lian-hua Han;Yafeng Zhou;Xin Zhao;Hong-Xia Li
影响因子：
--
作者：
Olivia Reetz;U. Strauss
通讯作者：
Olivia Reetz;U. Strauss
DOI：
10.1161/01.cir.0000087657.29184.9b
发表时间：
2003-09
期刊：
Circulation: Journal of the American Heart Association
影响因子：
--
作者：
Richard B. Thompson;S. Emani;B. Davis;E. J. van den Bos;Y. Morimoto;D. Craig;D. Glower;Doris A Taylor
通讯作者：
Richard B. Thompson;S. Emani;B. Davis;E. J. van den Bos;Y. Morimoto;D. Craig;D. Glower;Doris A Taylor