Interactions at the Silica-Peptide Interface: Influence of the Extent of Functionalization on the Conformational Ensemble.
Interactions at the Silica-Peptide Interface: Influence of the Extent of Functionalization on the Conformational Ensemble.
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二氧化硅-肽界面的相互作用：功能化程度对构象整体的影响。
DOI：
10.1021/acs.langmuir.8b00874
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
C. Perry
中科院分区：
文献类型：
--
作者：
Anna Sola;Monika Michaelis;Daniel J. Oliver;M. Roe;L. Colombi Ciacchi;H. Heinz;C. Perry
在这篇文章中，研究了二氧化硅粒径（28和210 nm）和表面化学（即，羟基、甲基或氨基）对肽结合反应的影响，特别强调官能化程度对结合的影响。详尽的表征的二氧化硅表面是至关重要的知识的化学和拓扑结构的固体表面的研究，因此，了解其对吸附和构象合奏的肽的影响。表面官能化的程度被证明是颗粒尺寸依赖性的，更高水平的3-氨基丙基官能度被获得较小的颗粒，而更高程度的甲基官能团被发现为较大的颗粒。我们表明，肽在水界面的相互作用不仅受到表面化学的影响，而且还通过观察到的肽吸附行为的“开关”的官能化程度，而圆二色谱显示的构象合奏的变化是独立的官能化程度。除了在二氧化硅-肽界面处的静电相互作用和氢键驱动相互作用之外，所获得的数据表明，更强的相互作用如疏水和/或共价相互作用可以缓和相互作用。从这项肽-矿物质研究中获得的见解提供了有关矿物质-肽相互作用机制的更全面的观点，这可能允许设计和合成具有特定应用特性的新型（纳米）材料。
In this contribution, the effect of silica particle size (28 and 210 nm) and surface chemistry (i.e., hydroxyl, methyl, or amino groups) on peptide binding response is studied with a specific emphasis on the effect of the extent of functionalization on binding. Exhaustive characterization of the silica surfaces was crucial for knowledge of the chemistry and topography of the solid surface under study and, thus, to understand their impact on adsorption and the conformational ensemble of the peptides. The extent of surface functionalization was shown to be particle-size dependent, a higher level of 3-aminopropyl functionality being obtained for smaller particles, whereas a higher degree of methyl group functionality was found for the larger particles. We demonstrated that peptide interactions at the aqueous interface were not only influenced by the surface chemistry but also by the extent of functionalization where a "switch" of peptide adsorption behavior was observed, whereas the changes in the conformational ensemble revealed by circular dichroism were independent of the extent of functionalization. In addition to electrostatic interactions and hydrogen bonding driving interaction at the silica-peptide interface, the data obtained suggested that stronger interactions such as hydrophobic and/or covalent interactions may moderate the interaction. The insights gained from this peptide-mineral study give a more comprehensive view of mechanisms concerning mineral-peptide interactions which may allow for the design and synthesis of novel (nano)materials with properties tailored for specific applications.
影响因子：
3.9
作者：
Puddu, Valeria;Perry, Carole C.
通讯作者：
Perry, Carole C.
影响因子：
2.9
作者：
Whitmore, Lee;Wallace, B. A.
通讯作者：
Wallace, B. A.
影响因子：
17.1
作者：
Puddu, Valeria;Perry, Carole C.
通讯作者：
Perry, Carole C.