The actin nucleator Cordon-bleu is required for development of motile cilia in zebrafish.
The actin nucleator Cordon-bleu is required for development of motile cilia in zebrafish.
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DOI：
10.1016/j.ydbio.2010.11.023
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发表时间：
2011-02-01
影响因子：
2.7
通讯作者：
Klingensmith J
中科院分区：
文献类型：
--
作者：
Ravanelli AM;Klingensmith J
Cordon-bleu（Cobl）基因在脊椎动物中广泛保守，在小鼠和鸡胚胎中具有发育调节的轴向和上皮表达。在体外，Cobl可以结合单体肌动蛋白和无分支肌动蛋白丝的成核形成，而在培养的细胞中，它可以调节肌动蛋白细胞骨架。然而，Cobl在体内的重要作用尚未确定。我们已经使用斑马鱼作为模型来评估胚胎发生中对Cobl的需求。我们发现cobl在斑马鱼器官发生过程中在纤毛上皮组织中表达丰富。Cobl蛋白富集在纤毛细胞的顶端结构域中，靠近顶端肌动蛋白帽。通过反义morpholinos减少cobl揭示了在诸如枯否氏囊泡和原肾等器官中的运动纤毛的发育中的重要作用。在库普弗囊泡中，Cobl的减少与顶端F-肌动蛋白的量的减少一致。因此，Cobl代表了一种分子活性，其将发育模式信号与局部细胞内细胞骨架动力学偶联以支持运动纤毛的形态发生。
The Cordon-bleu (Cobl) gene is widely conserved in vertebrates, with developmentally regulated axial and epithelial expression in mouse and chick embryos. In vitro, Cobl can bind monomeric actin and nucleate formation of unbranched actin filaments, while in cultured cells it can modulate the actin cytoskeleton. However, an essential role for Cobl in vivo has yet to be determined. We have used zebrafish as a model to assess the requirements for Cobl in embryogenesis. We find that cobl shows enriched expression in ciliated epithelial tissues during zebrafish organogenesis. Cobl protein is enriched in the apical domain of ciliated cells, in close proximity to the apical actin cap. Reduction of cobl by antisense morpholinos reveals an essential role in development of motile cilia in organs such as Kupffer's vesicle and the pronephros. In Kupffer's vesicle, the reduction in Cobl coincides with a reduction in the amount of apical F-actin. Thus, Cobl represents a molecular activity that couples developmental patterning signals with local intracellular cytoskeletal dynamics to support morphogenesis of motile cilia.
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影响因子：
11.8
作者：
Bershteyn, Marina;Atwood, Scott X.;Woo, Wei-Meng;Li, Mischa;Oro, Anthony E.
通讯作者：
Oro, Anthony E.
影响因子：
2.7
作者：
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通讯作者：
Klingensmith, J
影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
2004-07-01
影响因子：
3.9
作者：
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通讯作者：
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影响因子：
4
作者：
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通讯作者：
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