Influence of biosurfactant-producing strain Bacillus subtilis BS1 on the mycoremediation of soils contaminated with phenanthrene
Influence of biosurfactant-producing strain Bacillus subtilis BS1 on the mycoremediation of soils contaminated with phenanthrene
复制标题
生物表面活性剂生产菌株枯草芽孢杆菌 BS1 对菲污染土壤菌体修复的影响
DOI：
10.1016/j.ibiod.2012.09.002
复制
发表时间：
2012-11
期刊：
影响因子：
--
通讯作者：
Lijun Wu
中科院分区：
文献类型：
--
作者：
Xiang Xiao;Hao Chen;Chuncan Si;Lijun Wu
研究了生物表面活性剂产生菌枯草芽孢杆菌（Bacillus subtilis）BS 1与丛枝菌根真菌球囊霉（Glomus etunicatum）的生物相容性。接种BS 1能显著提高齿环格孢菌的萌发率和菌丝生长，但其代谢生物表面活性剂surfactin在高浓度下对齿环格孢菌的萌发有抑制作用。在离体条件下，菲胁迫抑制了桐花菇孢子萌发和菌丝生长，而接种BS 1可缓解菲胁迫，并显著促进萌发孢子的菌丝生长。采用盆栽试验研究了B. subtilis BS 1对不同浓度（0、50和250 mg kg −1）菲污染土壤的微生物修复效果。菌根定殖或接种BS 1提高了植株对菲胁迫的耐受性，增加了植株生物量。BS 1菌株分泌的生物表面活性剂显著提高了菲的溶解度，有利于菲在根际土壤和植物根系中的富集。结果表明，BS1和G.etunicatum共接种显著降低了土壤中菲的残留量，提高了土壤中过氧化氢酶和多酚氧化酶的活性。因此，将生物表面活性剂产生菌接种到真菌根际土壤中，可以作为一种潜在的生物技术手段来修复受多环芳烃污染的土壤。
In the present study, the biocompatibility between a biosurfactant-producing bacterial strain Bacillus subtilis BS1 and arbuscular mycorrhizal fungi Glomus etunicatum was investigated. Inoculation of BS1 could significantly increase the germination rate and hyphal development of G. etunicatum in vitro, although its metabolic biosurfactant, surfactin, displayed inhibitory effect at high concentrations. Exposed to the stress of phenanthrene inhibited the spore germination and hyphal development of G. etunicatum in vitro, but inoculation of BS1 was found to alleviate the stress of phenanthrene and remarkably promote the mycelium growth of germinated spores. The effect of B. subtilis BS1 on the mycoremediation of soils artificially contaminated with different levels of phenanthrene (0, 50 and 250mgkg−1) was investigated in pot experiments. Mycorrhizal colonization or BS1 inoculation improved the tolerance to stress of phenanthrene and increased the plant biomass. Biosurfactant secreted by BS1 strain considerably enhanced the solubility of phenanthrene, favoring its enrichment in rhizosphere soil and plant roots. The co-inoculation of BS1 and G. etunicatum significantly decreased the residual concentrations of phenanthrene in soil, and resulted in higher soil enzyme activities of catalase and polyphenol oxidase. Therefore, the inoculation of biosurfactant-producing strain to the mycorrhizosphere could be a potential biotechnological approach for the remediation of soil polluted with polycyclic aromatic hydrocarbons.
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DOI：
10.1016/j.ibiod.2011.04.001
发表时间：
2011-08-01
影响因子：
4.8
作者：
Bharali, P.;Das, S.;Thakur, A. J.
通讯作者：
Thakur, A. J.
生物表面活性剂和微生物的相互作用增强植物修复去除污染土壤中老化的多环芳烃
影响因子：
--
作者：
Chen, Ruirui;Liu, Weiwei;Lin, Xiangui;Li, Xuanzhen;Yin, Rui;Zhang, Jing
通讯作者：
Zhang, Jing
影响因子：
2.5
作者：
T. Pawlowska;D. Douds;I. Charvat
通讯作者：
T. Pawlowska;D. Douds;I. Charvat
影响因子：
4.8
作者：
C. Ruggeri;A. Franzetti;G. Bestetti;P. Caredda;Paolo La Colla;M. Pintus;S. Sergi;E. Tamburini
通讯作者：
C. Ruggeri;A. Franzetti;G. Bestetti;P. Caredda;Paolo La Colla;M. Pintus;S. Sergi;E. Tamburini
在非离子表面活性剂存在下植物加速菲和芘从水中的消散
影响因子：
8.8
作者：
Gao, YZ;Ling, WT;Wong, MH
通讯作者：
Wong, MH