Methodological comparison of two anti-ZAP-70 antibodies.
Methodological comparison of two anti-ZAP-70 antibodies.
复制标题
DOI：
10.1002/cyto.b.20591
复制
发表时间：
2011-09
影响因子：
3.4
通讯作者：
Marti, Gerald E.
中科院分区：
文献类型：
--
作者：
Degheidy, Heba A.;Venzon, David J.;Farooqui, Mohammed Z. H.;Abbasi, Fatima;Arthur, Diane C.;Wilson, Wyndham H.;Wiestner, Adrian;Stetler-Stevenson, M. A.;Marti, Gerald E.
ZAP-70表达是CLL中的分期独立预后标志物。然而，实验室间差异很大，既没有共识，也没有监管批准的方法。在45名未经治疗的CLL患者中比较了两种抗ZAP 70克隆（1E7.2和SBZAP）。评价了9种不同的ZAP-70表达分析方法：M1，确定阴性的同种型对照; M2，确定阳性的内部残留T细胞; M3，确定阳性的正常供体（ND）T细胞; M4，内部T细胞/克隆比率; M5，ND残留T细胞/克隆比率; M6，克隆/正常剩余B细胞比率; M7，克隆/ND B细胞比率; M8，CLL-Z评分; M9，改良CLL-Z评分。设计了整合1E7.2和SBZAP克隆的评分系统以分配ZAP-70表达。所选4种最高统计学显著性方法的相关系数如下（M1=0.71、M3=0.72、M7= 0.67和M9=0.64）。这四种方法用于生成综合评分。使用设计的评分系统，两种试剂对37/45份样品（82%）显示一致，8/45份（18%）显示两种克隆之一的不确定结果。使用评分系统解决了8份可疑样本中的7份。对每种试剂的9种分析方法中的4种进行了比较。使用两种独立的ZAP-70试剂增加了分析的可靠性，评分方法有助于解决不确定的结果。两种试剂、四种分析方法和评分方法的组合使用允许在44/45个测试样品（98%）中分配ZAP-70表达，并提高了这种重要的预后测定的性能。
ZAP-70 expression is a stage independent prognostic marker in CLL. However, inter-laboratory variation is large and there is neither a consensus nor regulatory approved methodology. Two anti-ZAP70 clones (1E7.2 and SBZAP) were compared in 45 untreated CLL patients. Nine different methods for ZAP-70 expression analysis were evaluated: M1, isotype control to determine negative; M2, internal residual T-cell to determine positive; M3, normal donor (ND) T-cell to determine positive; M4, internal T-cell/clone ratio; M5, ND residual T-cell/clone ratio; M6, clone/normal remaining B-cell ratio; M7, clone/ND B- cell ratio; M8, CLL- Z score; M9, modified CLL-Z score. A scoring system was designed integrating both 1E7.2 and SBZAP clones to assign ZAP-70 expression. The correlation coefficients for the four selected highest statistically significant methods were as follows (M1=0.71, M3=0.72, M7= 0.67, and M9=0.64). These four methods were used to generate a combined score. The two reagents showed agreement using the designed scoring system for 37/45 samples (82%) and 8/45 (18%) showed equivocal result with one of the two clones. Seven of the eight equivocal samples were resolved using the scoring system. Four of the nine methods of analysis were compared for each reagent. The use of two independent ZAP-70 reagents increases analytical certitude and the scoring method aids in the resolution of equivocal results. The combined use of two reagents, four methods of analysis and a scoring method allowed for assignment of ZAP-70 expression in 44/45 samples (98%) tested and improved performance of this important prognostic assay.
登录
查看更多内容
影响因子：
3.4
作者：
Kern, Wolfgang;Dicker, Frank;Haferlach, Torsten
通讯作者：
Haferlach, Torsten
影响因子：
158.5
作者：
Crespo, M;Bosch, F;Montserrat, E
通讯作者：
Montserrat, E
影响因子：
20.3
作者：
RAI, KR;SAWITSKY, A;PASTERNACK, BS
通讯作者：
PASTERNACK, BS
影响因子：
158.5
作者：
Rassenti, LZ;Huynh, L;Kipps, TJ
通讯作者：
Kipps, TJ
影响因子：
3.4
作者：
Shankey, T. Vincent;Forman, Meryl;Keeney, Mike
通讯作者：
Keeney, Mike