Over-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana.
Over-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana.
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KdSOC1基因过表达影响长寿花植株形态建成
DOI：
10.1038/s41598-017-04387-0
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发表时间：
2017-07-17
影响因子：
4.6
通讯作者：
Wang H
中科院分区：
文献类型：
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作者：
Zhu C;Wang L;Chen J;Liu C;Zeng H;Wang H
无性繁殖的kalanche daigremontiana沿着叶缘产生植株。本研究的目的是确定甘蓝中CONSTANS 1基因过表达抑制因子（KdSOC1）在植株形态发生过程中的功能。本研究在体外体细胞胚胎发生和植株形态发生过程中检测了KdSOC1基因在干细胞生态位的表达。破坏内源性生长素运输抑制了KdSOC1基因的反应。在体细胞胚胎发生的早期心脏阶段，KdSOC1基因的敲低导致子叶形成缺陷。KdSOC1基因的过表达（OE）导致植株分布不对称，植株数量减少，叶片变厚，维管纤维变厚。与野生型植物叶片相比，OE植物叶片中KdPIN1基因表达量和生长素含量均较高，说明KdSOC1基因可能影响生长素的分布和积累。DR5-GUS拟南芥报告系的KdSOC1基因OE导致体细胞胚发生不同阶段生长素响应模式异常。综上所述，KdSOC1基因OE可能改变植物生长素沿叶片边缘的分布和积累，从而启动植株的形成和分布，这对植株在各种环境条件下形成过程中的可塑性至关重要。
Kalanchoe daigremontiana reproduces asexually by producing plantlets along the leaf margin. The aim of this study was to identify the function of the SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 gene in Kalanchoe daigremontiana (KdSOC1) during plantlet morphogenesis. In this study, KdSOC1 gene expression was detected at stem cell niche during in vitro somatic embryogenesis and plantlet morphogenesis. Disrupting endogenous auxin transportation suppressed the KdSOC1 gene response. Knockdown of the KdSOC1 gene caused a defect in cotyledon formation during the early heart stage of somatic embryogenesis. Over-expression (OE) of the KdSOC1 gene resulted in asymmetric plantlet distribution, a reduced number of plantlets, thicker leaves, and thicker vascular fibers. Higher KdPIN1 gene expression and auxin content were found in OE plant compared to those of wild-type plant leaves, which indicated possible KdSOC1 gene role in affecting auxin distribution and accumulation. KdSOC1 gene OE in DR5-GUS Arabidopsis reporting lines resulted in an abnormal auxin response pattern during different stages of somatic embryogenesis. In summary, the KdSOC1 gene OE might alter auxin distribution and accumulation along leaf margin to initiate plantlet formation and distribution, which is crucial for plasticity during plantlet formation under various environmental conditions.
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影响因子：
7.2
作者：
Lee, Jungeun;Oh, Mijin;Lee, Ilha
通讯作者：
Lee, Ilha
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
4.6
作者：
Liu, Chang;Chen, Hongyan;Yu, Hao
通讯作者：
Yu, Hao
转基因烟草中银杏查尔酮合酶基因启动子的功能表征
影响因子：
0.4
作者：
Li, L. L.;Cheng, H.;Cao, F. L.
通讯作者：
Cao, F. L.
DOI：
10.1242/dev.117614
发表时间：
2015-07-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Gaillochet C;Lohmann JU
通讯作者：
Lohmann JU