Transposition of the retrotransposon MAGGY in heterologous species of filamentous fungi.
Transposition of the retrotransposon MAGGY in heterologous species of filamentous fungi.
复制标题
丝状真菌异源物种中反转录转座子 MAGGY 的转座。
作者：
H. Nakayashiki;K. Kiyotomi;Y. Tosa;S. Mayama
MAGGY是从稻瘟病菌稻瘟病菌（pyricaria grisea，远变形，Magnaporthe grisea）中分离出来的一种吉普赛样LTR反转录转座子。我们检测了最初不具有MAGGY元素的三种稻瘟病菌分离物（小麦、谷子和蟹草病原体）和两种异种丝状真菌（lagenarium Colletotrichum和zingiberi）中MAGGY的转位。MAGGY突变体的基因组南方分析表明，所有丝状真菌都发生了MAGGY的转位。相比之下，在含有513-bp逆转录酶结构域缺失的修饰MAGGY的任何转化子中均未观察到转位。当携带人工内含子的MAGGY衍生物被引入小麦稻瘟病菌和lagenarium小麦分离株时，观察到内含子丢失。这些结果表明，MAGGY可以在异源丝状真菌中进行自主rna介导的转位。不同种类真菌的转位频率不同。MAGGY在稻瘟病菌和姜叶病菌的小麦分离株中有活跃的转位，但在lagenarium中的转位似乎很少见。这是第一个证明真菌转座因子在异源宿主中的主动转座的报告。讨论了MAGGY作为丝状真菌遗传标记工具的可能性。
MAGGY is a gypsy-like LTR retrotransposon isolated from the blast fungus Pyricularia grisea (teleomorph, Magnaporthe grisea). We examined transposition of MAGGY in three P. grisea isolates (wheat, finger millet, and crabgrass pathogen), which did not originally possess a MAGGY element, and in two heterologous species of filamentous fungi, Colletotrichum lagenarium and P. zingiberi. Genomic Southern analysis of MAGGY transformants suggested that transposition of MAGGY occurred in all filamentous fungi tested. In contrast, no transposition was observed in any transformants with a modified MAGGY containing a 513-bp deletion in the reverse transcriptase domain. When a MAGGY derivative carrying an artificial intron was introduced into the wheat isolate of P. grisea and C. lagenarium, loss of the intron was observed. These results showed that MAGGY can undergo autonomous RNA-mediated transposition in heterologous filamentous fungi. The frequency of transposition differed among fungal species. MAGGY transposed actively in the wheat isolate of P. grisea and P. zingiberi, but transposition in C. lagenarium appeared to be rare. This is the first report that demonstrates active transposition of a fungal transposable element in heterologous hosts. Possible usage of MAGGY as a genetic tagging tool in filamentous fungi is discussed.
使用酵母中的 Ty 元件进行转座子标记。
影响因子：
3.3
作者：
Garfinkel,DJ;Mastrangelo,MF;Sanders,NJ;Shafer,BK;Strathern,JN
通讯作者：
Strathern,JN
马铃薯晚疫病病原体致病疫霉中存在 Ty1-copia 组逆转录转座子序列。
DOI：
10.1094/mpmi-9-0305
发表时间：
1996
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
Tooley,PW;Garfinkel,DJ
通讯作者：
Garfinkel,DJ