Physical methods for intracellular delivery: practical aspects from laboratory use to industrial-scale processing.
Physical methods for intracellular delivery: practical aspects from laboratory use to industrial-scale processing.
复制标题
DOI：
10.1177/2211068213494388
复制
发表时间：
2014-02
期刊：
影响因子：
--
通讯作者：
Fedorov AG
中科院分区：
文献类型：
--
作者：
Meacham JM;Durvasula K;Degertekin FL;Fedorov AG
有效的细胞内递送是所有处理规模的研究和治疗应用的重大障碍。长期以来，物理递送方法证明了将货物分子直接递送到细胞质或细胞核的能力，最常见的方法(显微注射、电穿孔和声波穿孔)背后的机制也得到了广泛的研究。在这篇综述中，我们讨论了已有的方法，以及新兴的技术(磁感应、光注入和联合模式)。除了操作原理和实施策略外，我们还讨论了各种体外、体外和体内平台的适用性和局限性。重要的是，我们对(1)不同细胞类型和输送的货物分子的治疗效果，(2)对不同处理规模(从单个细胞到大群体)的适应性，(3)自动化/与现有工作流程集成的适应性，以及(4)多路复用潜力和灵活性/适应性，以实现不同细胞类型的处理之间的快速转换，进行关键评估。现有技术通常不符合其中一个或多个标准；然而，微/纳米技术概念的引入以及互补方法的协同耦合(S)可以改善特定方法的性能和适用性，克服实际实施的障碍。为此，我们在审查运载系统发展的最新进展时强调这些战略。
Effective intracellular delivery is a significant impediment to research and therapeutic applications at all processing scales. Physical delivery methods have long demonstrated the ability to deliver cargo molecules directly to the cytoplasm or nucleus, and the mechanisms underlying the most common approaches (microinjection, electroporation, and sonoporation) have been extensively investigated. In this review, we discuss established approaches, as well as emerging techniques (magnetofection, optoinjection, and combined modalities). In addition to operating principles and implementation strategies, we address applicability and limitations of various in vitro, ex vivo, and in vivo platforms. Importantly, we perform critical assessments regarding (1) treatment efficacy with diverse cell types and delivered cargo molecules, (2) suitability to different processing scales (from single cell to large populations), (3) suitability for automation/integration with existing workflows, and (4) multiplexing potential and flexibility/adaptability to enable rapid changeover between treatments of varied cell types. Existing techniques typically fall short in one or more of these criteria; however, introduction of micro-/nanotechnology concepts, as well as synergistic coupling of complementary method(s), can improve performance and applicability of a particular approach, overcoming barriers to practical implementation. For this reason, we emphasize these strategies in examining recent advances in development of delivery systems.
登录
查看更多内容
影响因子：
7.4
作者：
Adamo, Andrea;Arione, Alessandro;Sharei, Armon;Jensen, Klavs F.
通讯作者：
Jensen, Klavs F.
DOI：
10.1016/0165-022x(88)90062-0
发表时间：
1988-08-01
影响因子：
--
作者：
ANSORGE, W;PEPPERKOK, R
通讯作者：
PEPPERKOK, R
影响因子：
3.2
作者：
Carugo, Dario;Ankrett, Dyan N.;Hill, Martyn
通讯作者：
Hill, Martyn
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
3.6
作者：
Chuah, Marinee K. L.;Collen, Desire;VandenDriessche, Thierry
通讯作者：
VandenDriessche, Thierry