Virulence gene profiles and molecular genetic characteristics of diarrheagenic Escherichia coli from a hospital in western China.
Virulence gene profiles and molecular genetic characteristics of diarrheagenic Escherichia coli from a hospital in western China.
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中国西部某医院致泻性大肠杆菌的毒力基因谱和分子遗传学特征。
DOI：
10.1186/s13099-018-0262-9
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发表时间：
2018
期刊：
影响因子：
4.2
通讯作者：
Ma Y
中科院分区：
文献类型：
--
作者：
Li D;Shen M;Xu Y;Liu C;Wang W;Wu J;Luo X;Jia X;Ma Y
致泻性大肠杆菌（DEC）是腹泻病最重要的病原之一。在这项研究中，我们调查了中国西部一家医院 DEC 的患病率、毒力基因谱、抗菌药物耐药性和分子遗传特征。 2015年至2016年从成都医学院第一附属医院收集110株大肠埃希菌临床分离株，采用微生物学方法、PCR、药敏试验、脉冲场凝胶电泳和多位点序列分型等方法。对6个DEC致病型标记基因的分子分析表明，110个大肠杆菌分离株中有13个（11.82%）是DEC，其中包括9个（8.18%）弥漫贴壁型大肠杆菌（DAEC）和4个（3.64%）肠聚集型大肠杆菌（EAEC）。粘附基因 fimC 和 fimH 存在于所有 DAEC 和 EAEC 分离株中。所有九个 DAEC 分离株都携带毒力基因 fyuA 和 irp2，四个 (44.44%) 还携带 hlyA 和 sat 基因。毒力基因 fyuA、irp2、cnf1、hlyA 和 sat 分别在 100%、100%、75%、50% 和 50% 的 EAEC 分离株中被发现。此外，所有 DEC 分离株均具有多重耐药性，并且具有较高的抗菌药物耐药性。分子遗传学表征表明，13 个 DEC 分离株分为 11 种脉冲场凝胶电泳模式和 10 种序列类型。据我们所知，本研究首次报告了中国西部地区的DEC，包括DAEC和EAEC。我们的分析鉴定了医院大肠杆菌中存在的毒力基因，表明它们在分离的 DEC 菌株发病机制中的作用。同时，分析揭示了 DEC 分离株的抗菌素耐药性模式。因此，DEC 菌株中的 DAEC 和 EAEC 由于其进化的致病性和抗菌素耐药性模式，应被视为对中国西部地区的人类构成重大风险。
Diarrheagenic Escherichia coli (DEC) is one of the most important etiological agents of diarrheal diseases. In this study we investigated the prevalence, virulence gene profiles, antimicrobial resistance, and molecular genetic characteristics of DEC at a hospital in western China. A total of 110 Escherichia coli clinical isolates were collected from the First Affiliated Hospital of Chengdu Medical College from 2015 to 2016. Microbiological methods, PCR, antimicrobial susceptibility test, pulsed-field gel electrophoresis and multilocus sequence typing were used in this study. Molecular analysis of six DEC pathotype marker genes showed that 13 of the 110 E. coli isolates (11.82%) were DEC including nine (8.18%) diffusely adherent Escherichia coli (DAEC) and four (3.64%) enteroaggregative Escherichia coli (EAEC). The adherence genes fimC and fimH were present in all DAEC and EAEC isolates. All nine DAEC isolates harbored the virulence genes fyuA and irp2 and four (44.44%) also carried the hlyA and sat genes. The virulence genes fyuA, irp2, cnf1, hlyA, and sat were found in 100%, 100%, 75%, 50%, and 50% of EAEC isolates, respectively. In addition, all DEC isolates were multidrug resistant and had high frequencies of antimicrobial resistance. Molecular genetic characterization showed that the 13 DEC isolates were divided into 11 pulsed-field gel electrophoresis patterns and 10 sequence types. To the best of our knowledge, this study provides the first report of DEC, including DAEC and EAEC, in western China. Our analyses identified the virulence genes present in E. coli from a hospital indicating their role in the isolated DEC strains’ pathogenesis. At the same time, the analyses revealed, the antimicrobial resistance pattern of the DEC isolates. Thus, DAEC and EAEC among the DEC strains should be considered a significant risk to humans in western China due to their evolved pathogenicity and antimicrobial resistance pattern.
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影响因子：
3.1
作者：
Boisen, Nadia;Struve, Carsten;Nataro, James P.
通讯作者：
Nataro, James P.
影响因子：
2.6
作者：
Bouzari, S;Oloomi, M;Oswald, E
通讯作者：
Oswald, E
DOI：
10.1007/s10096-009-0720-x
发表时间：
2009-08-01
影响因子：
4.5
作者：
Antikainen, J.;Tarkka, E.;Kirveskari, J.
通讯作者：
Kirveskari, J.
影响因子：
3.7
作者：
Bai X;Zhao A;Lan R;Xin Y;Xie H;Meng Q;Jin D;Yu B;Sun H;Lu S;Xu J;Xiong Y
通讯作者：
Xiong Y
影响因子：
5.4
作者：
Dean, Paul;Kenny, Brendan
通讯作者：
Kenny, Brendan