Shiga toxin-producing Escherichia coli in yaks (Bos grunniens) from the Qinghai-Tibetan Plateau, China.
Shiga toxin-producing Escherichia coli in yaks (Bos grunniens) from the Qinghai-Tibetan Plateau, China.
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DOI：
10.1371/journal.pone.0065537
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Xiong Y
中科院分区：
文献类型：
--
作者：
Bai X;Zhao A;Lan R;Xin Y;Xie H;Meng Q;Jin D;Yu B;Sun H;Lu S;Xu J;Xiong Y
产生志贺毒素（Stx）的大肠杆菌（STEC）被认为是引起公共卫生关注的重要人类病原体。许多动物都是 STEC 的来源。在这项研究中，我们确定了中国青藏高原牦牛 (Bos grunniens) 中 STEC 的发生和特征。 2012年6月至8月共采集728份牦牛粪便样本，经改良胰蛋白胨大豆肉汤富集后，通过TaqMan实时PCR筛选stx 1和stx 2基因的存在。在 138 个（18.96%）stx 1 和/或 stx 2 阳性样本中，通过培养分离证实 85 个（61.59%）样本中至少存在 1 个 STEC 分离株，从中回收了 128 个 STEC 分离株。所有 STEC 分离株均通过脉冲场凝胶电泳 (PFGE) 进行血清分型和基因分型，并确定是否存在 16 种已知毒力因子。在 128 个 STEC 分离株中鉴定出 15 个不同的 O 血清群和 36 个不同的 O:H 血清型，其中分别有 21 个和 4 个 O 和 H 抗原无法分型。这些 STEC 分离株中存在 1 种 stx 1 亚型 (stx 1a) 和 5 种 stx 2 亚型（stx 2a、stx 2b、stx 2c、stx 2d 和 stx 2g）。除了 lpfA O157/OI-141、lpfA O157/OI-154、lpfA O113、katP 和 toxB 均不存在外，筛选的其他毒力因子（eaeA、iha、efa1、saa、paa、cnf1、cnf2、astA、subA、exhA 和 espP）也不同程度地存在于128 个 STEC 分离株。除 5 个分离株外，PFGE 对所有菌株均成功，并将它们分为 67 种不同的 PFGE 模式。对于具有 2 个或更多分离株的 18 个血清型，相同血清型的分离株具有相同或密切相关的 PFGE 模式，证明了这些血清型的克隆性。该研究首次报道了从中国青藏高原牦牛（Bos grunniens）分离的STEC的发生和特征，扩展了STEC的遗传多样性和储存宿主范围。
Shiga toxin (Stx)-producing Escherichia coli (STEC) are recognized as important human pathogens of public health concern. Many animals are the sources of STEC. In this study we determined the occurrence and characteristics of the STEC in yaks (Bos grunniens) from the Qinghai-Tibetan plateau, China. A total of 728 yak fecal samples was collected from June to August, 2012 and was screened for the presence of the stx 1 and stx 2 genes by TaqMan real-time PCR after the sample was enriched in modified Tryptone Soya Broth. Of the 138 (18.96%) stx 1 and/or stx 2-positive samples, 85 (61.59%) were confirmed to have at least 1 STEC isolate present by culture isolation, from which 128 STEC isolates were recovered. All STEC isolates were serotyped, genotyped by pulsed-field gel electrophoresis (PFGE) and characterized for the presence of 16 known virulence factors. Fifteen different O serogroups and 36 different O:H serotypes were identified in the 128 STEC isolates with 21 and 4 untypable for the O and H antigens respectively. One stx 1 subtype (stx 1a) and 5 stx 2 subtypes (stx 2a, stx 2b, stx 2c, stx 2d and stx 2g) were present in these STEC isolates. Apart from lpfA O157/OI-141, lpfA O157/OI-154, lpfA O113, katP and toxB which were all absent, other virulence factors screened (eaeA, iha, efa1, saa, paa, cnf1, cnf2, astA, subA, exhA and espP) were variably present in the 128 STEC isolates. PFGE were successful for all except 5 isolates and separated them into 67 different PFGE patterns. For the 18 serotypes with 2 or more isolates, isolates of the same serotypes had the same or closely related PFGE patterns, demonstrating clonality of these serotypes. This study was the first report on occurrence and characteristics of STEC isolated from yaks (Bos grunniens) from the Qinghai-Tibetan plateau, China, and extended the genetic diversity and reservoir host range of STEC.
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影响因子：
9.4
作者：
Boerlin, P;McEwen, SA;Gyles, CL
通讯作者：
Gyles, CL
影响因子：
11.8
作者：
Grant, Juliana;Wendelboe, Aaron M.;Rolfs, Robert T.
通讯作者：
Rolfs, Robert T.
影响因子：
3.3
作者：
Auvray, Frederic;Dilasser, Francoise;Brugere, Hubert
通讯作者：
Brugere, Hubert
影响因子：
4.4
作者：
Coombes, Brian K.;Wickham, Mark E.;Karmali, Mohamed A.
通讯作者：
Karmali, Mohamed A.
影响因子：
3.1
作者：
Batisson, I;Guimond, MP;Harel, J
通讯作者：
Harel, J