Synthesis of sonicated fibrin nanoparticles that modulate fibrin clot polymerization and enhance angiogenic responses.
Synthesis of sonicated fibrin nanoparticles that modulate fibrin clot polymerization and enhance angiogenic responses.
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DOI：
10.1016/j.colsurfb.2021.111805
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Brown AC
中科院分区：
文献类型：
--
作者：
Roosa CA;Muhamed I;Young AT;Nellenbach K;Daniele MA;Ligler FS;Brown AC
当愈合过程被破坏并且伤口长期处于炎症阶段时，就会发生慢性伤口，从而导致严重的组织损伤和愈合结果不佳。临床使用的治疗方法，例如 FDA 批准的高密度纤维蛋白密封剂，不能为天然细胞增殖和随后的组织再生提供最佳环境。因此，需要在这些传统纤维蛋白散装凝胶疗法的范围之外进行新的治疗。我们之前开发了可流动的低密度纤维蛋白纳米颗粒，当与角质形成细胞生长因子结合时，可促进体内细胞迁移和上皮伤口闭合。在这里，我们报告了一种使用探针超声处理生成纤维蛋白纳米颗粒的新高通量方法，该方法比之前报道的微流体方法耗时更少，并研究了超声处理的纤维蛋白纳米颗粒（SFBN）在体外促进凝块形成和细胞迁移的能力。在没有外源凝血酶的情况下，SFBN 与纤维蛋白原结合时可以形成纤维蛋白凝胶，并且这些纤维蛋白凝块中的聚合速率和纤维密度可根据 SFBN 浓度进行调节。此外，用 SFBN 制成的纤维蛋白凝胶在体外血管生成萌芽测定中支持细胞迁移，这与伤口愈合相关。在这份报告中，我们表明 SFBN 可能是一种有前途的伤口愈合疗法，可以轻松地以可流动制剂的形式生产和输送。
Chronic wounds can occur when the healing process is disrupted and the wound remains in a prolonged inflammatory stage that leads to severe tissue damage and poor healing outcomes. Clinically used treatments, such as high density, FDA-approved fibrin sealants, do not provide an optimal environment for native cell proliferation and subsequent tissue regeneration. Therefore, new treatments outside the confines of these conventional fibrin bulk gel therapies are required. We have previously developed flowable, low-density fibrin nanoparticles that, when coupled to keratinocyte growth factor, promote cell migration and epithelial wound closure in vivo. Here, we report a new high throughput method for generating the fibrin nanoparticles using probe sonication, which is less time intensive than the previously reported microfluidic method, and investigate the ability of the sonicated fibrin nanoparticles (SFBN) to promote clot formation and cell migration in vitro. The SFBNs can form a fibrin gel when combined with fibrinogen in the absence of exogenous thrombin, and the polymerization rate and fiber density in these fibrin clots is tunable based on SFBN concentration. Furthermore, fibrin gels made with SFBNs support cell migration in an in vitro angiogenic sprouting assay, which is relevant for wound healing. In this report, we show that SFBNs may be a promising wound healing therapy that can be easily produced and delivered in a flowable formulation.
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影响因子：
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作者：
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DOI：
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