Genomic organization of a cellulase gene family in Phanerochaete chrysosporium
Genomic organization of a cellulase gene family in Phanerochaete chrysosporium
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黄孢原平革菌纤维素酶基因家族的基因组结构
DOI：
10.1007/bf00310904
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发表时间：
1992
期刊：
影响因子：
2.5
通讯作者：
D. Cullen
中科院分区：
文献类型：
--
作者：
S. Covert;J. Bolduc;D. Cullen
Southern杂交和核苷酸序列分析表明，黄孢原毛平革菌BKM-F-1767基因组中至少含有6个与里氏木霉纤维二糖水解酶I基因（cbh 1）具有高度同源性的基因。利用脉冲场凝胶电泳分离黄孢原毛平革菌染色体，发现6个纤维素酶基因至少与3条不同的染色体杂交，其中一条染色体是二态的。这些基因的结构与另一株黄孢原毛平革菌ME 446相似。很明显，与T. reesei，研究最充分的纤维素分解真菌，P. chrysosporium包含一个复杂的cbh 1样基因家族。
Southern blot and nucleotide sequence analysis of Phanerochaete chrysosporium BKM-F-1767 genomic clones indicate that this wood-degrading fungus contains at least six genes with significant homology to the Trichoderma reesei cellobiohydrolase I gene (cbh1). Using pulsed-field gel electrophoresis to separate P. chrysosporium chromosomes, the six cellulase genes were found to hybridize to at least three different chromosomes, one of which is dimorphic. The organization of these genes was similar in another P. chrysosporium strain, ME 446. It is clear that, unlike T. reesei, the most well-studied cellulolytic fungus, P. chrysosporium contains a complex, cbh1-like gene family.
DOI：
10.1073/pnas.86.16.6260
发表时间：
1989
影响因子：
11.1
作者：
Brody,H;Carbon,J
通讯作者：
Carbon,J
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs