Electrokinetically-driven transport of DNA through focused ion beam milled nanofluidic channels.
Electrokinetically-driven transport of DNA through focused ion beam milled nanofluidic channels.
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DOI：
10.1021/ac303074f
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发表时间：
2013-01-15
影响因子：
7.4
通讯作者：
Ramsey, J. Michael
中科院分区：
文献类型：
--
作者：
Menard, Laurent D.;Ramsey, J. Michael
描述了双链 λ 噬菌体 DNA 通过聚焦离子束 (FIB) 研磨纳米通道的电泳驱动运输。纳米通道的临界尺寸（宽度和深度）分别对应于 DNA 持久长度的 0.5 倍、1 倍和 2 倍，即 25 纳米、50 纳米和 100 纳米。测量驱动传输所需的阈值场强、线程迁移率和传输迁移率作为纳米通道尺寸的函数。随着纳米通道尺寸的减小，易位的熵势垒增加，运输变得更加受限。限制的平衡模型提供了一个框架来理解观察到的趋势，尽管实验的动态性质导致了与理论的显着偏差。还证明，使用动态壁涂层来抑制电渗流可以对传输动力学产生重大影响，从而可能混淆熵的贡献。通过 FIB 研磨纳米通道的非间歇性 DNA 传输表明它们非常适合在纳米流体装置中使用。我们期望对此处报告的动态传输特性的理解将有助于将 FIB 研磨的纳米通道纳入单分子和整体分析的设备中。
The electrophoretically-driven transport of double-stranded λ-phage DNA through focused ion beam (FIB) milled nanochannels is described. Nanochannels were fabricated having critical dimensions (width and depth) corresponding to 0.5×, 1×, and 2× the DNA persistence length – or 25 nm, 50 nm, and 100 nm, respectively. The threshold field strength required to drive transport, the threading mobility, and the transport mobility were measured as a function of nanochannel size. As the nanochannel dimensions decreased, the entropic barrier to translocation increased and transport became more constrained. Equilibrium models of confinement provide a framework in which to understand the observed trends, although the dynamic nature of the experiments resulted in significant deviations from theory. It was also demonstrated that the use of dynamic wall coatings for the purpose of electroosmotic flow suppression can have a significant impact on transport dynamics that may obfuscate entropic contributions. The non-intermittent DNA transport through the FIB milled nanochannels demonstrates that they are well suited for use in nanofluidic devices. We expect that an understanding of the dynamic transport properties reported here will facilitate the incorporation of FIB-milled nanochannels in devices for single molecule and ensemble analyses.
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