Differential DNA methylation in discrete developmental stages of the parasitic nematode Trichinella spiralis.
Differential DNA methylation in discrete developmental stages of the parasitic nematode Trichinella spiralis.
复制标题
寄生线虫旋毛虫离散发育阶段的差异 DNA 甲基化
DOI：
10.1186/gb-2012-13-10-r100
复制
发表时间：
2012-10-17
期刊：
影响因子：
12.3
通讯作者：
Liu M
中科院分区：
文献类型：
--
作者：
Gao F;Liu X;Wu XP;Wang XL;Gong D;Lu H;Xia Y;Song Y;Wang J;Du J;Liu S;Han X;Tang Y;Yang H;Jin Q;Zhang X;Liu M
背景
DNA甲基化在多种条件下调节基因表达中起着重要作用，因此已被假设为寄生线虫生命周期阶段之间转换的基础。然而，到目前为止，在秀丽隐杆线虫的任何发育阶段都没有检测到5'-胞嘧啶甲基化。鉴于高分辨率甲基化检测方法的新可用性，现在可以进行寄生线虫中生命周期甲基化的调查。
结果
在这里，使用MethylC-seq，我们提出了第一项研究，以确认寄生线虫旋毛虫中存在DNA甲基化，我们表征了这种食源性传染性人类病原体的三个生命周期阶段的甲基化。我们观察到在从新生到成熟阶段的过渡期间DNA甲基化急剧增加，并且我们进一步鉴定了在生命周期阶段之间显示DNA甲基化状态变化的寄生虫相关基因。
结论
我们的数据有助于了解一种重要的人类寄生虫发生的发育变化，并提出了靶向DNA甲基化过程可能是开发治疗方法以阻止感染的有用策略的可能性。此外，我们的结论是，DNA甲基化是一种机制，生活史转变的T。spiralis提出了一个问题，即这是否也可能是任何其他后生动物的情况。最后，我们的工作构成了第一份报告，据我们所知，DNA甲基化的线虫，促使重新评估门，这种表观遗传标记被认为是缺席。
Background
DNA methylation plays an essential role in regulating gene expression under a variety of conditions and it has therefore been hypothesized to underlie the transitions between life cycle stages in parasitic nematodes. So far, however, 5'-cytosine methylation has not been detected during any developmental stage of the nematode Caenorhabditis elegans. Given the new availability of high-resolution methylation detection methods, an investigation of life cycle methylation in a parasitic nematode can now be carried out.
Results
Here, using MethylC-seq, we present the first study to confirm the existence of DNA methylation in the parasitic nematode Trichinella spiralis, and we characterize the methylomes of the three life-cycle stages of this food-borne infectious human pathogen. We observe a drastic increase in DNA methylation during the transition from the new born to mature stage, and we further identify parasitism-related genes that show changes in DNA methylation status between life cycle stages.
Conclusions
Our data contribute to the understanding of the developmental changes that occur in an important human parasite, and raises the possibility that targeting DNA methylation processes may be a useful strategy in developing therapeutics to impede infection. In addition, our conclusion that DNA methylation is a mechanism for life cycle transition in T. spiralis prompts the question of whether this may also be the case in any other metazoans. Finally, our work constitutes the first report, to our knowledge, of DNA methylation in a nematode, prompting a re-evaluation of phyla in which this epigenetic mark was thought to be absent.
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