Lyophilization of semiconducting polymer dot bioconjugates.
Lyophilization of semiconducting polymer dot bioconjugates.
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DOI：
10.1021/ac4007123
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发表时间：
2013-05-07
影响因子：
7.4
通讯作者：
Chiu, Daniel T.
中科院分区：
文献类型：
--
作者：
Sun, Wei;Ye, Fangmao;Gallina, Maria E.;Yu, Jiangbo;Wu, Changfeng;Chiu, Daniel T.
半导体聚合点生物结合物是一类新型的超亮荧光探针。在这里，我们报告了一种冷冻干燥PDot生物结合物的方法，使它们在储存过程中成功地保持其光学性质、胶体稳定性和细胞靶向能力。我们发现，当在10%蔗糖存在的情况下进行冷冻干燥时，复水的Pdot生物结合物没有表现出任何聚集的迹象，并且表现出与冷冻干燥前相同的流体直径。冷冻干燥的Pdots的白度至少和冷冻干燥前一样好，但在某些情况下，冷冻干燥的Pdots的量子产率奇怪地出现了提高。最后，通过使用流式细胞术，我们证明了冻干的pDot生物结合物保留了它们的生物靶向性，并且能够有效地标记细胞；事实上，由pFBT组成的冻干pDot生物结合物在−80°C下储存6个月，标记的细胞比相同但未冻干的pDot生物结合物标记的细胞亮度高~22%。这些结果表明，冷冻干燥可能是储存和运输Pdots生物结合物的首选方法，这是确保Pdots在生物医学研究中广泛采用的一个重要的实用考虑。
Semiconducting polymer dot (Pdot) bioconjugates are a new class of ultrabright fluorescent probes. Here, we report a procedure for lyophilizing Pdot bioconjugates so that they successfully retain their optical properties, colloidal stability, and cell-targeting capability during storage. We found that when Pdot bioconjugates were lyophilized in the presence of 10% sucrose, the rehydrated Pdot bioconjugates did not show any signs of aggregation and exhibited the same hydrodynamic diameters as before lyophilization. The brightness of the lyophilized Pdots was at least as good as before lyophilization, but in some cases, the quantum yield of lyophilized Pdots curiously showed an improvement. Finally, by using flow cytometry, we demonstrated that lyophilized Pdot bioconjugates retained their biological targeting properties and were able to effectively label cells; in fact, cells labeled with lyophilized Pdot bioconjugates composed of PFBT, which were stored for 6 months at −80 °C, were ~ 22% brighter than those labeled with identical but unlyophilized Pdot bioconjugates. These results indicate lyophilization may be a preferred approach for storing and shipping Pdot bioconjugates, which is an important practical consideration for ensuring Pdots are widely adopted in biomedical research.
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DOI：
10.1039/c2cc16486h
发表时间：
2012-02-07
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Ye F;Wu C;Jin Y;Wang M;Chan YH;Yu J;Sun W;Hayden S;Chiu DT
通讯作者：
Chiu DT
影响因子：
15
作者：
Wu C;Schneider T;Zeigler M;Yu J;Schiro PG;Burnham DR;McNeill JD;Chiu DT
通讯作者：
Chiu DT
影响因子：
29.4
作者：
Yu, Jiangbo;Wu, Changfeng;Zhang, Xuanjun;Ye, Fangmao;Gallina, Maria Elena;Rong, Yu;Wu, I-Che;Sun, Wei;Chan, Yang-Hsiang;Chiu, Daniel T.
通讯作者：
Chiu, Daniel T.
影响因子：
10.8
作者：
AUSBORN, M;SCHREIER, H;NUHN, P
通讯作者：
NUHN, P
影响因子：
16.6
作者：
Wu, Changfeng;Hansen, Stacey J.;Hou, Qiong;Yu, Jiangbo;Zeigler, Maxwell;Jin, Yuhui;Burnham, Daniel R.;McNeill, Jason D.;Olson, James M.;Chiu, Daniel T.
通讯作者：
Chiu, Daniel T.