Bioconjugation of ultrabright semiconducting polymer dots for specific cellular targeting.
Bioconjugation of ultrabright semiconducting polymer dots for specific cellular targeting.
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DOI：
10.1021/ja107196s
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发表时间：
2010-11-03
影响因子：
15
通讯作者：
Chiu DT
中科院分区：
文献类型：
--
作者：
Wu C;Schneider T;Zeigler M;Yu J;Schiro PG;Burnham DR;McNeill JD;Chiu DT
半导体聚合物点（Pdot）代表了一类新的用于生物成像的超亮荧光探针。它们表现出几个重要的特性，实验要求在体外和体内荧光研究，如它们的高亮度，快速发射速率，优良的光稳定性，不闪烁，无毒的功能。然而，控制Pdot的表面化学和生物缀合一直是一个具有挑战性的问题，阻碍了它们在生物研究中的广泛应用。在这里，我们报告了一个简单而强大的共轭方法，克服了这一挑战。我们的Pdot功能化策略是基于将异质聚合物链捕获到单个点中，由纳米颗粒形成期间的疏水相互作用驱动。少量带有官能团的两亲聚合物与大多数半导体聚合物共缩合，以修饰和官能化纳米颗粒表面，用于随后共价缀合至生物分子，例如链霉亲和素和免疫球蛋白G（IgG）。Pdot生物缀合物可以有效地和特异性地标记细胞靶标，例如人乳腺癌细胞中的细胞表面标记物，而没有任何可检测的非特异性结合。单粒子成像、细胞成像和流式细胞术实验表明，与Alexa染料和量子点探针相比，Pdot的荧光亮度高得多。这些超亮纳米粒子的成功生物共轭为将多功能半导体聚合物应用于现代生物学和生物医学中的各种荧光测量提供了新的机会。
Semiconducting polymer dots (Pdots) represent a new class of ultrabright fluorescent probes for biological imaging. They exhibit several important characteristics for experimentally demanding in vitro and in vivo fluorescence studies, such as their high brightness, fast emission rate, excellent photostability, non-blinking, and non-toxic feature. However, controlling the surface chemistry and bioconjugation of Pdots has been a challenging problem that prevented their widespread applications in biological studies. Here, we report a facile yet powerful conjugation method that overcomes this challenge. Our strategy for Pdot functionalization is based on entrapping heterogeneous polymer chains into a single dot, driven by hydrophobic interactions during nanoparticle formation. A small amount of amphiphilic polymer bearing functional groups is co-condensed with the majority of semiconducting polymers to modify and functionalize the nanoparticle surface for subsequent covalent conjugation to biomolecules, such as streptavidin and immunoglobulin G (IgG). The Pdot bioconjugates can effectively and specifically label cellular targets, such as cell surface marker in human breast cancer cells, without any detectable non-specific binding. Single-particle imaging, cellular imaging, and flow cytometry experiments indicate a much higher fluorescence brightness of Pdots compared to those of Alexa dye and quantum dot probes. The successful bioconjugation of these ultrabright nanoparticles presents a novel opportunity to apply versatile semiconducting polymers to various fluorescence measurements in modern biology and biomedicine.
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影响因子：
16.6
作者：
Moon, Joong Ho;McDaniel, William;Hancock, Lawrence E.
通讯作者：
Hancock, Lawrence E.
影响因子：
15
作者：
Howes, Philip;Green, Mark;Hughes, Mary
通讯作者：
Hughes, Mary
影响因子：
56.9
作者：
Bruchez, M;Moronne, M;Alivisatos, AP
通讯作者：
Alivisatos, AP
DOI：
10.1073/pnas.1132025100
发表时间：
2003-05-27
影响因子：
11.1
作者：
Fan, CH;Wang, S;Heeger, AJ
通讯作者：
Heeger, AJ
影响因子：
3.7
作者：
Wu, Changfeng;Zheng, Yueli;McNeill, Jason
通讯作者：
McNeill, Jason