Stem cell plasticity revisited: the continuum marrow model and phenotypic changes mediated by microvesicles.
Stem cell plasticity revisited: the continuum marrow model and phenotypic changes mediated by microvesicles.
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DOI：
10.1016/j.exphem.2010.03.021
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发表时间：
2010-07
影响因子：
2.6
通讯作者：
Aliotta, Jason M.
中科院分区：
文献类型：
--
作者：
Quesenberry, Peter J.;Dooner, Mark S.;Aliotta, Jason M.
骨髓造血干细胞的表型由细胞周期状态和微泡进入干细胞决定。干细胞群体基于细胞周期转运而不断变化，因此只能在群体基础上定义。骨髓干细胞的纯化仅解决了这些群体的异质性。当研究整个骨髓时，长期再生的干细胞处于活跃的细胞周期中。然而，当研究高度纯化的干细胞时，这些细胞似乎处于休眠状态，存在一些变异性。因此，对纯化干细胞的研究本质上是误导的。损伤增强的组织来源的微泡影响不同细胞类别的表型。我们提出，先前描述的干细胞可塑性是由于微泡调制。我们进一步提出了一个干细胞群体模型，其中单个细胞表型不断变化，但群体表型相对稳定。这反过来又受到微囊泡和微环境影响的调节。
The phenotype of marrow hematopoietic stem cells is determined by cell cycle state and microvesicle entry into the stem cells. The stem cell population is continually changing based on cell cycle transit and thus can only be defined on a population basis. Purification of marrow stem cells only addresses the heterogeneity of these populations. When whole marrow is studied, the long-term repopulating stem cells are in active cell cycle. However, with some variability, when highly purified stem cells are studied, the cells appear to be dormant. Thus, the study of purified stem cells is intrinsically misleading. Tissue-derived microvesicles enhanced by injury effect the phenotype of different cell classes. We propose that previously described stem cell plasticity is due to microvesicle modulation. We further propose a stem cell population model in which the individual cell phenotypes continually changes, but the population phenotype is relatively stable. This, in turn, is modulated by microvesicle and microenvironmental influences.
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影响因子：
64.8
作者：
Alvarez-Dolado, M;Pardal, R;Alvarez-Buylla, A
通讯作者：
Alvarez-Buylla, A
DOI：
10.1007/s004290050237
发表时间：
1999-05-01
期刊：
ANATOMY AND EMBRYOLOGY
影响因子：
--
作者：
Bittner, RE;Schöfer, C;Wachtler, F
通讯作者：
Wachtler, F
DOI：
10.1089/152581602321080574
发表时间：
2002-12-01
期刊：
JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH
影响因子：
--
作者：
Cerny, J;Dooner, M;Quesenberry, P
通讯作者：
Quesenberry, P
影响因子：
64.5
作者：
Adolfsson, J;Månsson, R;Jacobsen, SEW
通讯作者：
Jacobsen, SEW
影响因子：
20.3
作者：
Arber, C;BitMansour, A;Brown, JMY
通讯作者：
Brown, JMY