Receptor-mediated endocytosis generates nanomechanical force reflective of ligand identity and cellular property.
Receptor-mediated endocytosis generates nanomechanical force reflective of ligand identity and cellular property.
复制标题
受体介导的内吞作用产生反映配体身份和细胞特性的纳米机械力。
DOI：
10.1002/jcp.26400
复制
发表时间：
2018-08
影响因子：
5.6
通讯作者：
Gao N
中科院分区：
文献类型：
--
作者：
Zhang X;Ren J;Wang J;Li S;Zou Q;Gao N
Whether environmental (thermal, chemical, and nutrient) signals generate quantifiable, nanoscale, mechanophysical changes in the cellular plasma membrane has not been well elucidated. Assessment of such mechanophysical properties of plasma membrane may shed lights on fundamental cellular process. Atomic force microscopic (AFM) measurement of the mechanical properties of live cells was hampered by the difficulty in accounting for the effects of the cantilever motion and the associated hydrodynamic force on the mechanical measurement. These challenges have been addressed in our recently developed control-based AFM nanomechanical measurement protocol, which enables a fast, noninvasive, broadband measurement of the real-time changes in plasma membrane elasticity in live cells. Here we show using this newly developed AFM platform that the plasma membrane of live mammalian cells exhibits a constant and quantifiable nanomechanical property, the membrane elasticity. This mechanical property sensitively changes in response to environmental factors, such as the thermal, chemical, and growth factor stimuli. We demonstrate that different chemical inhibitors of endocytosis elicit distinct changes in plasma membrane elastic modulus reflecting their specific molecular actions on the lipid configuration or the endocytic machinery. Interestingly, two different growth factors, EGF and Wnt3a, elicited distinct elastic force profiles revealed by AFM at the plasma membrane during receptor-mediated endocytosis. By applying this platform to genetically modified cells, we uncovered a previously unknown contribution of Cdc42, a key component of the cellular trafficking network, to EGF-stimulated endocytosis at plasma membrane. Together, this nanomechanical AFM study establishes an important foundation that is expandable and adaptable for investigation of cellular membrane evolution in response to various key extracellular signals.
登录
查看更多内容
影响因子：
5.5
作者：
Bernardino de la Serna J;Schütz GJ;Eggeling C;Cebecauer M
通讯作者：
Cebecauer M
影响因子：
3.4
作者：
Frankel, DJ;Pfeiffer, JR;Burns, AR
通讯作者：
Burns, AR
影响因子：
7.3
作者：
Hill, T;Odell, LR;McCluskey, A
通讯作者：
McCluskey, A
影响因子：
4.8
作者：
Ares, Gustavo R.;Ortiz, Pablo A.
通讯作者：
Ortiz, Pablo A.
影响因子：
4.5
作者：
Day, Charles A.;Baetz, Nicholas W.;Kenworthy, Anne K.
通讯作者：
Kenworthy, Anne K.