Physical and chemical evolution of sugar beet pulp during aqueous ammonia pretreatment and its impacts on the recalcitrance of cellulose
Physical and chemical evolution of sugar beet pulp during aqueous ammonia pretreatment and its impacts on the recalcitrance of cellulose
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氨水预处理甜菜浆物理化学演化及其对纤维素不顺应性的影响
DOI：
10.1016/j.biombioe.2022.106678
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发表时间：
2023-01
影响因子：
6
通讯作者：
Guanhua Li
中科院分区：
文献类型：
--
作者：
Qianqian Qian;Lin Yuan;Yufei Chen;Huiting Xue;Guanhua Li
Recognition and deconstruction of biomass recalcitrance are essential steps in fuel ethanol production. Aqueous ammonia (AA) is an efficient pretreatment to improve the enzymatic digestibility of sugar beet pulp (SBP), but the precise mechanism is still ambiguous. Multidimensional methods, e.g., physical properties, wet chemistry compositions, and glycome profiling were integrated to elucidate the impacts of AA pretreatment on recalcitrance of SBP semi-quantitatively. Additionally, the variations in enzyme adsorption and cell wall morphology during the enzymatic hydrolysis were systematically observed. The results confirmed that the constitution, association, and distribution of glycan epitopes within cell walls significantly correlated to recalcitrance. Specifically, the arabinogalactan and rhamnogalacturonan epitopes negatively correlated to the recalcitrance. And xylans only bound to cellulose contributed to recalcitrance. Dislocation and fragmentation of cell walls through sequential removal of several glycan epitopes could facilitate enzymatic digestibility, in terms of enzyme adsorption, reducing sugar yields, and enzymatic saccharification behaviors. Whereas, cell corners seemed to be highly resistant to enzymatic digestion due to some gathered epitopes. The maximum reducing sugar yields ranging from 437.7 to 441.48 mg/g were obtained from the pretreated SBP (3, 5, and 7 h), which nearly increased by 87% compared to the control. Selecting a proper pretreatment to precisely strip out the vital glycan epitopes might be an economically feasible strategy in fuel ethanol production.
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DOI：
10.1073/pnas.1912354116
发表时间：
2019-11-05
影响因子：
11.1
作者：
Novy, Vera;Aissa, Kevin;Saddler, Jack
通讯作者：
Saddler, Jack
漆酶预处理麦秆：理化特性和酶水解动力学的影响
影响因子：
6.3
作者：
Deng, Zhichao;Xia, Ao;Fu, Qian
通讯作者：
Fu, Qian
影响因子：
6.3
作者：
Sabbadin F;Pesante G;Elias L;Besser K;Li Y;Steele-King C;Stark M;Rathbone DA;Dowle AA;Bates R;Shipway JR;Cragg SM;Bruce NC;McQueen-Mason SJ
通讯作者：
McQueen-Mason SJ
影响因子：
5.9
作者：
Hansen, Mads A. T.;Ahl, Louise I.;Felby, Claus
通讯作者：
Felby, Claus
通过机械活化和金属盐的协同预处理克服生物质顽抗性以增强木质纤维素的酶转化
影响因子：
6.3
作者：
Yanjuan Zhang;Min Huang;Jianmei Su;Huayu Hu;Mei Yang;Zuqiang Huang;Dong Chen;Juan Wu;Zhenfei Feng
通讯作者：
Zhenfei Feng