Imaging PEG-like nanoprobes in tumor, transient ischemia, and inflammatory disease models.
Imaging PEG-like nanoprobes in tumor, transient ischemia, and inflammatory disease models.
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肿瘤、短暂性缺血和炎症疾病模型中的类 PEG 纳米探针成像。
DOI：
10.1021/acs.bioconjchem.5b00213
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发表时间：
2015
影响因子：
4.7
通讯作者：
Josephson,Lee
中科院分区：
文献类型：
--
作者：
Wilks,MosesQ;Normandin,MarcD;Yuan,Hushan;Cho,Hoonsung;Guo,Yanyan;Herisson,Fanny;Ayata,Cenk;Wooten,DustinW;ElFakhri,Georges;Josephson,Lee
铁螯合剂去铁胺（DFO），批准用于治疗铁过载，已被检查作为一种治疗在各种条件下，铁可能加剧。为了评估携带DFO的PEG样纳米探针（DFO-PN）作为治疗剂的潜力，我们确定了它们在正常小鼠中的药代动力学（PK），并对它们在肿瘤模型和短暂脑缺血和炎症模型中的积累进行了成像。DFO-PN由连接至Lys-Cys支架的DFO、Cy5.5和PEG（5 kDa或30 kDa）组成。通过PET、表面荧光和荧光显微镜对[89 Zr]：DFO-PN（10）（30 kDa PEG，直径10 nm）的肿瘤摄取进行成像。DFO-PN（10）被肿瘤细胞（荧光显微镜）和培养细胞（通过FACS）内化。[89 Zr]：DFO-PN（4.3）（5 kDa PEG，直径4.3 nm）浓缩在切口处产生炎症，但在短暂脑缺血部位不产生炎症。DFO-PN是DFO的荧光、PK可调形式，可作为抗肿瘤或抗炎剂进行研究。
The iron chelator deferoxamine (DFO), approved for the treatment of iron overload, has been examined as a therapeutic in a variety of conditions which iron may exacerbate. To evaluate the potential of DFO-bearing PEG-like nanoprobes (DFO-PNs) as therapeutics, we determined their pharmacokinetics (PK) in normal mice, and imaged their accumulation in a tumor model and in models of transient brain ischemia and inflammation. DFO-PNs consist of a DFO, a Cy5.5, and PEG (5 kDa or 30 kDa) attached to Lys-Cys scaffold. Tumor uptake of a [89Zr]:DFO-PN(10) (30 kDa PEG, diameter 10 nm) was imaged by PET, surface fluorescence, and fluorescence microscopy. DFO-PN(10) was internalized by tumor cells (fluorescence microscopy) and by cultured cells (by FACS). [89Zr]:DFO-PN(4.3) (5 kDa PEG, diameter 4.3 nm) concentrated at incision generated inflammations but not at sites of transient brain ischemia. DFO-PNs are fluorescent, PK tunable forms of DFO that might be investigated as antitumor or anti-inflammatory agents.
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影响因子：
4.7
作者：
Pauli, Jutta;Licha, Kai;Resch-Genger, Ute
通讯作者：
Resch-Genger, Ute
影响因子：
15
作者：
Guo, Yanyan;Yuan, Hushan;Rice, William L.;Kumar, Anand T. N.;Goergen, Craig J.;Jokivarsi, Kimmo;Josephson, Lee
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Josephson, Lee
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作者：
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影响因子：
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作者：
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Kyle Bickel
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影响因子：
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作者：
D. Richardson;P. Ponka;E. Baker
通讯作者：
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