Estrogen receptor-α and -β expression in microvascular endothelial cells and smooth muscle cells of myometrium and leiomyoma
Estrogen receptor-α and -β expression in microvascular endothelial cells and smooth muscle cells of myometrium and leiomyoma
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子宫肌层和平滑肌瘤微血管内皮细胞和平滑肌细胞中雌激素受体-α和-β的表达
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
P. Rogers
中科院分区：
文献类型：
--
作者：
C. Gargett;K. Bucak;M. Zaitseva;S. Chu;N. Taylor;P. Fuller;P. Rogers
两种雌激素受体ERα和ERβ可能在纤维瘤发展的病理生理学中起作用。在子宫肌层和平滑肌瘤（纤维瘤）组织中检测到它们，但表达ERα和ERβ的细胞类型尚未确定。在人类内皮细胞中也检测到了ER。本研究的目的是确定子宫肌层微血管内皮细胞（MEC）是否表达ERα和ERβ，比较MEC与子宫肌层平滑肌细胞（SMC）的ERα/ERβ表达，并确定ERα/ERβ在9对子宫肌层和子宫肌瘤的MEC和SMC中的差异表达。采用RT-PCR（检测ERα和ERβ）和Western blotting（仅检测ERα），我们证明所有培养的早期传代子宫肌层和纤维样平滑肌细胞（>99%纯度）表达ERα而不表达ERβ，而子宫肌层和纤维样MEC（>99% CD 31）组成性表达ERβ。然而，子宫肌层和纤维瘤MEC均显示ERα的可变表达，约60%的MEC样本表达ERα。虽然大多数（6/9）来自子宫肌层和肌瘤对的MEC显示出相同的ERα表达模式，但3/9对显示出不一致的ERα表达。结果表明，ERα和ERβ在人子宫肌层和子宫肌瘤的SMC和MEC中表达差异。由于ERα和ERβ介导相反的转录活性，雌激素对肌瘤生长和发育的任何影响都可能是复杂的，可能涉及SMC和MEC。
The two estrogen receptors, ERα and ERβ, are likely to have roles in the pathophysiology of fibroid development. They have been detected in myometrial and leiomyoma (fibroid) tissue, but the cell types expressing ERα and ERβ have not been determined. ERs have also been detected in human endothelial cells. The aims of the present study were to determine whether pure populations of myometrial microvascular endothelial cells (MEC) express ERα and ERβ, to compare MEC ERα/ERβ expression with that of pure populations of myometrial smooth muscle cells (SMC) and to determine if ERα/ERβ are differentially expressed in MEC and SMC of myometrium and fibroids from nine paired samples. Using RT–PCR (for ERα and ERβ) and Western blotting (for ERα only), we demonstrated that all cultures of early passage myometrial and fibroid SMC (>99% pure) expressed ERα but not ERβ, while myometrial and fibroid MEC (>99% CD31) constitutively expressed ERβ. However, both myometrial and fibroid MEC showed variable expression of ERα, with ~60% of MEC samples expressing ERα. While the majority (6/9) of MEC from myometrial and fibroid pairs demonstrated the same pattern of ERα expression, 3/9 pairs showed discordant ERα expression. These results show that ERα and ERβ are differentially expressed in SMC and MEC of human myometrium and fibroids. Since ERα and ERβ mediate opposing transcriptional activities, any effect of estrogen on the growth and development of fibroids is likely to be complex and may involve both SMC and MEC.
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足月时人子宫平滑肌中雌激素受体β表达增加。
影响因子：
5.8
作者：
Wu,JJ;Geimonen,E;Andersen,J
通讯作者：
Andersen,J
影响因子：
37.8
作者：
Venkov, CD;Rankin, AB;Vaughan, DE
通讯作者：
Vaughan, DE
影响因子：
37.8
作者：
MORALES, DE;MCGOWAN, KA;SCHNAPER, HW
通讯作者：
SCHNAPER, HW
DOI：
10.1016/s0002-9149(02)02405-0
发表时间：
2002-06
期刊：
The New England journal of medicine
影响因子：
--
作者：
M. Mendelsohn;R. Karas
通讯作者：
M. Mendelsohn;R. Karas