Conserved elements of the RAM signaling pathway establish cell polarity in the basidiomycete Cryptococcus neoformans in a divergent fashion from other fungi.
Conserved elements of the RAM signaling pathway establish cell polarity in the basidiomycete Cryptococcus neoformans in a divergent fashion from other fungi.
复制标题
RAM 信号通路的保守元件以与其他真菌不同的方式在担子菌新型隐球菌中建立细胞极性。
DOI：
10.1091/mbc.e06-02-0125
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发表时间：
2006
影响因子：
3.3
通讯作者：
Idnurm,Alexander
中科院分区：
文献类型：
--
作者：
Walton,FeliciaJ;Heitman,Joseph;Idnurm,Alexander
In eukaryotes the complex processes of development, differentiation, and proliferation require carefully orchestrated changes in cellular morphology. Single-celled eukaryotes provide tractable models for the elucidation of signaling pathways involved in morphogenesis. Here we describe a pathway regulating cell polarization and separation in the human pathogenic fungusCryptococcus neoformans. An insertional mutagenesis screen identified roles for theARF1, CAP60, NDH1,KIC1, CBK1, SOG2, andTAO3genes in establishing normal colony morphology.ARF1andCAP60are also required for capsule production, a virulence factor, andARF1confers resistance to the antifungal fluconazole.KIC1, CBK1, SOG2, andTAO3are homologues of genes conserved in other eukaryotes; inSaccharomyces cerevisiaethey constitute components of the RAM (regulation ofAce2p activity and cellularmorphogenesis) signaling pathway. A targeted deletion of a fifth component of RAM (MOB2) conferred identical phenotypes tokic1, cbk1, sog2,ortao3mutations. Characterization of these genes inC. neoformansrevealed unique features of the RAM pathway in this organism. Loss of any of these genes caused constitutive hyperpolarization instead of the loss of polarity seen inS. cerevisiae. Furthermore, sensitivity to the drugs FK506 and cyclosporin A demonstrates that the RAM pathway acts in parallel with the protein phosphatase calcineurin inC. neoformansbut not inS. cerevisiae. These results indicate that conserved signaling pathways serve both similar and divergent cellular roles in morphogenesis in these divergent organisms.
通道密度与阻断剂相关的变化。
DOI：
--
发表时间：
1990
期刊：
The Journal of General Physiology
影响因子：
--
作者：
S. I. Helman;Lynn M. Baxendale
通讯作者：
Lynn M. Baxendale
影响因子：
56.9
作者：
LINDEMANN, B;VANDRIESSCHE, W
通讯作者：
VANDRIESSCHE, W