Escherichia coli O157:H7 F9 Fimbriae Recognize Plant Xyloglucan and Elicit a Response in Arabidopsis thaliana.
Escherichia coli O157:H7 F9 Fimbriae Recognize Plant Xyloglucan and Elicit a Response in Arabidopsis thaliana.
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大肠杆菌 O157:H7 F9 菌毛识别植物木葡聚糖并在拟南芥中引发反应。
DOI：
10.3390/ijms21249720
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发表时间：
2020-12-19
影响因子：
5.6
通讯作者：
Holden NJ
中科院分区：
文献类型：
--
作者：
Holmes A;Rossez Y;Wright KM;Hedley PE;Morris J;Willats WGT;Holden NJ
新鲜农产品往往是肠出血性大肠杆菌（EHEC）爆发的来源。菌毛是参与细胞间附着和表面定殖的细胞外结构。已经显示F9（Fml）菌毛在低于37 °C的温度下表达，这意味着功能超出哺乳动物宿主。我们证明，F9菌毛识别植物细胞壁半纤维素，特别是半乳糖基化侧链的木葡聚糖，使用聚糖阵列。E.表达F9菌毛的大肠杆菌对菠菜半纤维素提取物和组织的粘附具有积极的优势，菠菜半纤维素提取物和组织具有LM24和LM25抗体识别的半乳糖基化寡糖。由于菌毛是具有分子模式的多聚体结构，我们研究了F9菌毛是否能在模式植物拟南芥中诱导转录反应，并与鞭毛和另一种菌毛类型E.大肠杆菌普通菌毛（ECP）的DNA微阵列。F9诱导了435个基因的差异表达，包括参与植物防御反应的基因。F9在环境相关温度下的表达及其对植物木葡聚糖的识别增加了EHEC可用于开发植物生态位的粘附素套件。
Fresh produce is often a source of enterohaemorrhagic Escherichia coli (EHEC) outbreaks. Fimbriae are extracellular structures involved in cell-to-cell attachment and surface colonisation. F9 (Fml) fimbriae have been shown to be expressed at temperatures lower than 37 °C, implying a function beyond the mammalian host. We demonstrate that F9 fimbriae recognize plant cell wall hemicellulose, specifically galactosylated side chains of xyloglucan, using glycan arrays. E. coli expressing F9 fimbriae had a positive advantage for adherence to spinach hemicellulose extract and tissues, which have galactosylated oligosaccharides as recognized by LM24 and LM25 antibodies. As fimbriae are multimeric structures with a molecular pattern, we investigated whether F9 fimbriae could induce a transcriptional response in model plant Arabidopsis thaliana, compared with flagella and another fimbrial type, E. coli common pilus (ECP), using DNA microarrays. F9 induced the differential expression of 435 genes, including genes involved in the plant defence response. The expression of F9 at environmentally relevant temperatures and its recognition of plant xyloglucan adds to the suite of adhesins EHEC has available to exploit the plant niche.
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影响因子：
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