Xenograft Skin Model to Manipulate Human Immune Responses In Vivo.
Xenograft Skin Model to Manipulate Human Immune Responses In Vivo.
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DOI：
10.3791/64040
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发表时间：
2022-06-29
期刊：
影响因子：
--
通讯作者：
Lowe MM
中科院分区：
文献类型：
--
作者：
Moss MI;Pauli M;Moreau JM;Cohen JN;Rosenblum MD;Lowe MM
人类皮肤异种移植模型是将人类供体皮肤移植到免疫缺陷小鼠宿主上，是皮肤免疫学转化研究的重要选择。小鼠和人类的皮肤在解剖结构和免疫细胞组成上有很大的不同。因此，传统的小鼠模型在皮肤病学研究和药物发现方面存在局限性。然而，成功的异种移植在技术上是具有挑战性的，并且需要最佳的标本和小鼠移植部位准备来保证移植物和宿主的存活。本方案提供了一种优化的人类皮肤移植到小鼠身上的技术，并讨论了下游实验目标的必要考虑。本报告描述了人体供体皮肤样本的适当准备，手术装置的组装，小鼠和手术部位的准备，皮肤移植和术后监测。坚持这些方法可以使异种移植物在术后维持6周以上。由于工程控制、无菌技术以及术前和术后调理的发展，下面概述的技术使移植效率达到最高。异种移植模型的适当性能导致长寿命的人类皮肤移植样品，用于人体皮肤的实验表征和体内化合物的临床前测试。
The human skin xenograft model, in which human donor skin is transplanted onto an immunodeficient mouse host, is an important option for translational research in skin immunology. Murine and human skin differ substantially in anatomy and immune cell composition. Therefore, traditional mouse models have limitations for dermatological research and drug discovery. However, successful xenotransplants are technically challenging and require optimal specimen and mouse graft site preparation for graft and host survival. The present protocol provides an optimized technique for transplanting human skin onto mice and discusses necessary considerations for downstream experimental aims. This report describes the appropriate preparation of a human donor skin sample, assembly of a surgical setup, mouse and surgical site preparation, skin transplantation, and post-surgical monitoring. Adherence to these methods allows for maintenance of xenografts for over 6 weeks post-surgery. The techniques outlined below allow maximum grafting efficiency due to the development of engineering controls, sterile technique, and pre- and post-surgical conditioning. Appropriate performance of the xenograft model results in long-lived human skin graft samples for experimental characterization of human skin and preclinical testing of compounds in vivo.
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影响因子：
4.4
作者：
Souci L;Denesvre C
通讯作者：
Denesvre C
DOI：
10.1111/wrr.12847
发表时间：
2020-11
期刊：
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
影响因子：
--
作者：
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通讯作者：
Gibson ALF
影响因子：
15.9
作者：
Rodriguez, Robert Sanchez;Pauli, Mariela L.;Rosenblum, Michael D.
通讯作者：
Rosenblum, Michael D.
影响因子：
5.5
作者：
Sun H;Zhang YX;Li YM
通讯作者：
Li YM
影响因子：
6.2
作者：
Racki WJ;Covassin L;Brehm M;Pino S;Ignotz R;Dunn R;Laning J;Graves SK;Rossini AA;Shultz LD;Greiner DL
通讯作者：
Greiner DL