MtCAS31 Aids Symbiotic Nitrogen Fixation by Protecting the Leghemoglobin MtLb120-1 Under Drought Stress in Medicago truncatula.
MtCAS31 Aids Symbiotic Nitrogen Fixation by Protecting the Leghemoglobin MtLb120-1 Under Drought Stress in Medicago truncatula.
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MtCAS31 通过保护蒺藜苜蓿干旱胁迫下的豆血红蛋白 MtLb120-1 来帮助共生固氮
DOI：
10.3389/fpls.2018.00633
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发表时间：
2018
影响因子：
5.6
通讯作者：
Wang T
中科院分区：
文献类型：
--
作者：
Li X;Feng H;Wen J;Dong J;Wang T
豆科植物根瘤共生固氮（SNF）向农田注入数百万吨氮素，并在缺氮条件下为非豆科作物提供氨。在植物生长发育过程中，干旱、盐胁迫、冷胁迫、热胁迫等环境胁迫是不可避免的。这就提出了一个有趣的问题，即豆科植物如何应对环境压力和SNF。在干旱胁迫下，脱水蛋白积累，具有蛋白质保护和渗透作用。在本研究中，我们发现脱水酶MtCAS31（冷驯化特异性31）在干旱胁迫下的短叶苜蓿SNF中起作用。我们发现MtCAS31在结节中表达，并与豆血红蛋白MtLb120-1相互作用。两种蛋白之间的相互作用保护MtLb120-1在体内热应激下不变性。与野生型相比，cas31突变体在脱水条件下表现出较低的氮酶活性、较低的ATP/ADP比率、较高的结节衰老基因表达和较高的淀粉质体积累。结果表明，MtCAS31可以保护MtLb120-1免受干旱胁迫的伤害。我们发现了干旱胁迫下脱水剂在SNF中的新功能，丰富了对脱水剂分子机制的认识。
Symbiotic nitrogen fixation (SNF) in legume root nodules injects millions of tons of nitrogen into agricultural lands and provides ammonia to non-legume crops under N-deficient conditions. During plant growth and development, environmental stresses, such as drought, salt, cold, and heat stress are unavoidable. This raises an interesting question as to how the legumes cope with the environmental stress along with SNF. Under drought stress, dehydrin proteins are accumulated, which function as protein protector and osmotic substances. In this study, we found that the dehydrin MtCAS31 (cold-acclimation-specific 31) functions in SNF in Medicago truncatula during drought stress. We found that MtCAS31 is expressed in nodules and interacts with leghemoglobin MtLb120-1. The interaction between the two proteins protects MtLb120-1 from denaturation under thermal stress in vivo. Compared to wild type, cas31 mutants display a lower nitrogenase activity, a lower ATP/ADP ratio, higher expression of nodule senescence genes and higher accumulation of amyloplasts under dehydration conditions. The results suggested that MtCAS31 protects MtLb120-1 from the damage of drought stress. We identified a new function for dehydrins in SNF under drought stress, which enriches the understanding of the molecular mechanism of dehydrins.
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