The JUN oncoprotein, a vertebrate transcription factor, activates transcription in yeast
The JUN oncoprotein, a vertebrate transcription factor, activates transcription in yeast
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JUN 癌蛋白是一种脊椎动物转录因子，可激活酵母中的转录
作者：
K. Struhl
从酵母到哺乳动物的真核生物中RNA聚合酶II的转录激活具有许多共同特征，如增强子元件、TATA元件和特异性结合启动子DNA的激活蛋白（综述见参考文献（1，2））。导致鸡肉瘤的JUN癌蛋白3与GCN 4的DNA结合结构域显示出显著的同源性，GCN 4是一种刺激氨基酸生物合成基因转录的酵母蛋白4。GCN 4和JUN蛋白结合相同的DNA序列5，共有ATGA（C/G）TCAT（参考文献6），尽管DNA结合结构域在氨基酸序列上仅45%相同。JUN蛋白几乎肯定代表正常AP-1转录因子7的致癌版本，表明酵母和脊椎动物激活蛋白之间的进化关系。在这里，我证明了JUN通过其自身或异源DNA结合结构域有效地激活酵母中的转录。与酵母激活蛋白的情况一样，JUN的转录刺激需要不同于DNA结合结构域的酸性激活区域。酵母和脊椎动物转录因子之间的功能相似性强烈表明真核生物转录激活的分子机制的基本相似性。
Transcriptional activation of RNA polymerase II in eukaryotic organisms ranging from yeasts to mammals has many common features such as enhancer elements, TATA elements, and activator proteins that bind specifically to promoter DNA (reviewed in refs (1, 2). The JUN oncoprotein, which causes sarcomas in chickens3, shows significant homology to the DNA-binding domain of GCN4, a yeast protein that stimulates transcription of the amino acid biosynthetic genes4. The GCN4 and JUN proteins bind the same DNA sequences5, consensus ATGA(C/G)TCAT (ref. 6), even though the DNA-binding domains are only 45% identical in amino acid sequence. The JUN protein almost certainly represents the oncogenic version of the normal AP-1 transcription factor7, sug-gesting an evolutionary relationship between yeast and vertebrate activator proteins. Here, I demonstrate that JUN efficiently activates transcription in yeast either through its own or a heterologous DNA-binding domain. As is the case for yeast activator proteins, transcriptional stimulation by JUN requires an acidic activation region distinct from the DNA-binding domain. The functional interchangeability between yeast and vertebrate transcription factors strongly suggests a basic similarity in the molecular mechanism of eukaryotic transcriptional activation.
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DOI：
10.1073/pnas.84.10.3316
发表时间：
1987-05
影响因子：
11.1
作者：
P. Vogt;T. Bos;R. Doolittle
通讯作者：
P. Vogt;T. Bos;R. Doolittle
DOI：
10.1073/pnas.84.9.2848
发表时间：
1987-05-01
影响因子：
11.1
作者：
MAKI, Y;BOS, TJ;VOGT, PK
通讯作者：
VOGT, PK
影响因子：
56.9
作者：
BOHMANN, D;BOS, TJ;TJIAN, R
通讯作者：
TJIAN, R
影响因子：
56.9
作者：
HILL, DE;HOPE, IA;STRUHL, K
通讯作者：
STRUHL, K
影响因子：
11.4
作者：
HOPE, IA;STRUHL, K
通讯作者：
STRUHL, K