Crystallization and preliminary X-ray diffraction analysis of human adenovirus.
Crystallization and preliminary X-ray diffraction analysis of human adenovirus.
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DOI：
10.1016/j.virol.2010.03.028
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发表时间：
2010-06-20
期刊：
影响因子：
3.7
通讯作者：
Nemerow GR
中科院分区：
文献类型：
--
作者：
Reddy VS;Natchiar SK;Gritton L;Mullen TM;Stewart PL;Nemerow GR
复制缺陷和有条件复制腺病毒（AdV）载体目前被用于约25%的人类基因转移临床试验。不幸的是，由于缺乏准确的结构信息，矢量发展的进展受到阻碍。在这里，我们描述了HAdV5矢量的结晶和初步的x射线衍射分析，该矢量显示了源自HAdV35的短柔性纤维。Ad35F晶体生长在100 mM HEPES pH 7.0, 200 mM Ca(OAc)2, 14% PEG 550 MME， 15%甘油100 mM Tris-HCl 8.5中。新鲜生长的晶体在同步加速器源下衍射分辨率为4.5 Å，弱衍射分辨率为3.5 Å。hav晶体属于空间群P1，晶胞参数a=854.03 Å， b=855.17 Å， c=865.24 Å， α=119.57°，β=91.71°，γ=118.08°，晶胞内只有一个粒子。S自旋转和锁定旋转函数分析可以确定粒子的取向。分子置换、密度修饰和相扩展方法被用于结构测定。
Replication-defective and conditionally replicating adenovirus (AdV) vectors are currently being utilized in ~25% of human gene transfer clinical trials. Unfortunately, progress in vector development has been hindered by a lack of accurate structural information. Here we describe the crystallization and preliminary X-ray diffraction analysis of a HAdV5 vector that displays a short flexible fiber derived from HAdV35. Crystals of Ad35F were grown in 100 mM HEPES pH 7.0, 200 mM Ca(OAc)2, 14% PEG 550 MME, 15% glycerol in 100 mM Tris-HCl 8.5. Freshly grown crystals diffracted well to 4.5 Å resolution and weakly to 3.5 Å at synchrotron sources. HAdV crystals belong to space group P1 with unit cell parameters a=854.03 Å, b=855.17 Å, c=865.24 Å, α=119.57°, β=91.71°, γ=118.08° with a single particle in the unit cell. S elf-rotation and locked-rotation function analysis allowed the determination of the particle orientation. Molecular replacement, density modification and phase-extension procedures are being employed for structure determination.
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DOI：
10.1107/s0907444905036693
发表时间：
2006-01-01
影响因子：
2.2
作者：
Evans, P
通讯作者：
Evans, P
影响因子：
64.5
作者：
STEWART, PL;BURNETT, RM;FULLER, SD
通讯作者：
FULLER, SD
影响因子：
12.4
作者：
Rux, JJ;Burnett, RM
通讯作者：
Burnett, RM
影响因子：
11.4
作者：
Fabry, CMS;Rosa-Calatrava, M;Schoehn, G
通讯作者：
Schoehn, G
影响因子：
64.5
作者：
CHOW, LT;GELINAS, RE;ROBERTS, RJ
通讯作者：
ROBERTS, RJ