3D printed microfluidic devices with integrated versatile and reusable electrodes.
3D printed microfluidic devices with integrated versatile and reusable electrodes.
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DOI：
10.1039/c4lc00171k
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发表时间：
2014-06-21
期刊：
影响因子：
6.1
通讯作者：
Spence DM
中科院分区：
文献类型：
--
作者：
Erkal JL;Selimovic A;Gross BC;Lockwood SY;Walton EL;McNamara S;Martin RS;Spence DM
我们报告了两种可用于电化学检测的3D打印设备。在这两种情况下，电极被容纳在市售的基于聚合物的配件中，使得各种电极材料（铂、铂黑、碳、金、银）可以容易地添加到印刷在装置上的螺纹接收端口;这实现了实验设计的模块式方法，其中电极是可移除的并且可以容易地重新抛光以在暴露于生物样品之后重复使用。第一个打印设备代表一个微流体平台，具有500 × 500 μm通道和螺纹接收端口，允许集成聚醚醚酮（PEEK）螺母包裹的玻璃碳或铂黑（Pt-黑）电极，分别用于多巴胺和一氧化氮（NO）检测。嵌入的1 mm玻碳电极对多巴胺的检测限（LOD）为500 nM，对25-500 μM浓度的线性响应（R2= 0.99）。当玻碳电极涂有0.05%的Nafion，显着排除亚硝酸盐时，观察到的信号相比，从等摩尔注射多巴胺。当使用Pt/Pt黑电极和来自NO气体的标准品进行流动注射分析时，在宽浓度范围（7.6 - 190 μM）内获得线性相关性（R2 = 0.99），NO的LOD为1 μM。第二个应用展示了一种3D打印的流体设备，该设备允许收集生物相关的分析物三磷酸腺苷（ATP），同时测量释放刺激（减少的氧浓度）。低氧样品（4.76 ± 0.53 ppm氧）释放的ATP是常氧样品（8.22 ± 0.60 ppm氧）的2.37 ± 0.37倍。重要的是，本文报告的结果验证了使用3D打印作为制造技术的可重复性和可转移性，因为在研究完成期间，设备和电极在实验室之间多次移动。
We report two 3D printed devices that can be used for electrochemical detection. In both cases, the electrode is housed in commercially available, polymer-based fittings so that the various electrode materials (platinum, platinum black, carbon, gold, silver) can be easily added to a threaded receiving port printed on the device; this enables a module-like approach to the experimental design, where the electrodes are removable and can be easily repolished for reuse after exposure to biological samples. The first printed device represents a microfluidic platform with a 500 × 500 μm channel and a threaded receiving port to allow integration of either polyetheretherketone (PEEK) nut-encased glassy carbon or platinum black (Pt-black) electrodes for dopamine and nitric oxide (NO) detection, respectively. The embedded 1 mm glassy carbon electrode had a limit of detection (LOD) of 500 nM for dopamine and a linear response (R2= 0.99) for concentrations between 25-500 μM. When the glassy carbon electrode was coated with 0.05% Nafion, significant exclusion of nitrite was observed when compared to signal obtained from equimolar injections of dopamine. When using flow injection analysis with a Pt/Pt-black electrode and standards derived from NO gas, a linear correlation (R2 = 0.99) over a wide range of concentrations (7.6 - 190 μM) was obtained, with the LOD for NO being 1 μM. The second application showcases a 3D printed fluidic device that allows collection of the biologically relevant analyte adenosine triphosphate (ATP) while simultaneously measuring the release stimulus (reduced oxygen concentration). The hypoxic sample (4.76 ± 0.53 ppm oxygen) released 2.37 ± 0.37 times more ATP than the normoxic sample (8.22 ± 0.60 ppm oxygen). Importantly, the results reported here verify the reproducible and transferable nature of using 3D printing as a fabrication technique, as devices and electrodes were moved between labs multiple times during completion of the study.
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影响因子：
2.7
作者：
Dragone V;Sans V;Rosnes MH;Kitson PJ;Cronin L
通讯作者：
Cronin L
DOI：
10.1039/c2an36168j
发表时间：
2013-01-07
期刊：
The Analyst
影响因子：
--
作者：
Johnson AS;Anderson KB;Halpin ST;Kirkpatrick DC;Spence DM;Martin RS
通讯作者：
Martin RS
影响因子：
4.2
作者：
Kovarik, ML;Torrence, NJ;Martin, RS
通讯作者：
Martin, RS
DOI：
10.1039/c2an36171j
发表时间：
2013-01-07
期刊：
The Analyst
影响因子：
--
作者：
Anderson KB;Halpin ST;Johnson AS;Martin RS;Spence DM
通讯作者：
Spence DM
影响因子：
7.4
作者：
Gross, Bethany C.;Erkal, Jayda L.;Spence, Dana M.
通讯作者：
Spence, Dana M.